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FITC Mouse Anti-Human CD61
FITC Mouse Anti-Human CD61

Flow cytometric analysis of CD61 expression on Rhesus macaque (Macaca mulatta) peripheral blood platelets. Platelets were stained with either FITC Mouse Anti-Human CD61 (Cat. No. 555753/561913/557291; solid line histogram) or FITC Mouse IgG1, κ Isotype Control (Cat. No. 551954). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable platelets. Flow cytometry was performed on a BD FACScan™ system.

Flow cytometric analysis of CD61 expression on Rhesus macaque (Macaca mulatta) peripheral blood platelets. Platelets were stained with either FITC Mouse Anti-Human CD61 (Cat. No. 555753/561913/557291; solid line histogram) or FITC Mouse IgG1, κ Isotype Control (Cat. No. 551954). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable platelets. Flow cytometry was performed on a BD FACScan™ system.

Product Details
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BD Pharmingen™
Integrin β3; Integrin beta-3; GP3A; GPIIIa; ITGB3; ITB3
Rhesus, Cynomolgus, Baboon (QC Testing), Human, Dog, Cow (Tested in Development)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
20 µl
III 866; IV P83; V T-124
3690
AB_396619
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
557291 Rev. 7
Antibody Details
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VI-PL2

The VI-PL2 monoclonal antibody specifically binds to CD61. CD61 is a 105 kDa transmembrane glycoprotein that is also known as integrin β3 and platelet glycoprotein IIIa (GPIIIa or GP3A). It is expressed on platelets, megakaryocytes, osteoclasts and endothelia. Integrin β3 associates with gpIIa (CD41) to form the CD41/CD61 complex which mediates platelet adhesion and aggregation. CD61 also associates with CD51 to form the CD51/CD61 complex (vitronectin receptor). CD61 appears to bind to fibrinogen, fibronectin, vWF, vitronectin, and thrombospondin to mediate cell adhesion.

557291 Rev. 7
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
557291 Rev.7
Citations & References
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Development References (3)

  1. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
  2. Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002.
  3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
557291 Rev. 7

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.