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FITC Mouse Anti-Human β2-Microglobulin
FITC Mouse Anti-Human β2-Microglobulin
Profile of peripheral blood lymphocytes analyzed by flow cytometry
FITC Mouse Anti-Human β2-Microglobulin
Multiparameter flow cytometric analysis of β2-Microglobulin expression on human peripheral blood leucocyte populations. Human whole blood was stained with either FITC Mouse IgM, κ Isotype Control (Cat. No.555583; Left Plot) or FITC Mouse Anti-Human β2-Microglobulin (Cat. No. 551338; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plots showing correlated expression of β2-Microglobulin [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scattering characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Profile of peripheral blood lymphocytes analyzed by flow cytometry
Multiparameter flow cytometric analysis of β2-Microglobulin expression on human peripheral blood leucocyte populations. Human whole blood was stained with either FITC Mouse IgM, κ Isotype Control (Cat. No.555583; Left Plot) or FITC Mouse Anti-Human β2-Microglobulin (Cat. No. 551338; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plots showing correlated expression of β2-Microglobulin [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scattering characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgM, κ
Flow cytometry (Routinely Tested)
20 µl
N/A
567
AB_394153
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
551338 Rev. 5
Antibody Details
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TÜ99

β2-microglobulin is the 12 kDa β chain which noncovalently associates with the 44 kDa α chain to form the HLA Class I antigen complex. This antibody reacts with free or complexed β2-microglobulin. TÜ99 does not react with β2-microglobulin of other species. This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections using TUF pretreatment.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

551338 Rev. 5
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
551338 Rev.5
Citations & References
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Development References (3)

  1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  2. Desoye G, Dohr GA, Motter W, et al. Lack of HLA class I and class II antigens on human preimplantation embryos. J Immunol. 1988; 140(12):4157-4159. (Biology). View Reference
  3. Dohr GA, Motter W, Leitinger S, et al. Lack of expression of HLA [corrected] class I and class II molecules on the human oocyte. J Immunol. 1987; 138(11):3766-3770. (Biology). View Reference
551338 Rev. 5

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.