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561996Image1.png

Two-color analysis of the expression of γδ TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with PE Rat anti-Mouse CD3 Molecular Complex (Cat. No. 555275) and FITC Hamster anti-Mouse γδ T-Cell Receptor (Cat. No. 561996/553177, right panel). Fluorescence dot plots were derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.

561996Image1.png Green-Cap-Blue-Label.png

Two-color analysis of the expression of γδ TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with PE Rat anti-Mouse CD3 Molecular Complex (Cat. No. 555275) and FITC Hamster anti-Mouse γδ T-Cell Receptor (Cat. No. 561996/553177, right panel). Fluorescence dot plots were derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.

Product Details
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BD Pharmingen™
Tcrd; T-cell receptor delta chain; Tcr delta
Mouse (QC Testing)
Armenian Hamster IgG2, κ
C57BL/6 Mouse Intestinal Intraepithelial Lymphocytes
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_10895796
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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Recommended Assay Procedures

For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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561996 Rev. 2
Antibody Details
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GL3

The GL3 antibody reacts with a common epitope of the δ chain of the T-cell Receptor (TCR) complex on γδ TCR-expressing T lymphocytes and NK-T cells of all mouse strains tested. It does not react with αβ TCR-bearing T cells. In the mouse, cells expressing the γδ TCR are found in the thymus, intestinal epithelium, epidermis, dermis, pulmonsry epithelium, peritoneum, liver, and peripheral lymphoid organs.

561996 Rev. 2
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
FITC
494 nm
518 nm
561996 Rev.2
Citations & References
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View product citations for antibody "561996" on CiteAb

Development References (11)

  1. Goodman T, LeCorre R, Lefrancois L. A T-cell receptor gamma delta-specific monoclonal antibody detects a V gamma 5 region polymorphism. Immunogenetics. 1992; 35(1):65-68. (Clone-specific: Flow cytometry). View Reference
  2. Goodman T, Lefrancois L. Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function. J Exp Med. 1989; 170(5):1569-1581. (Clone-specific: Flow cytometry). View Reference
  3. Kaufmann SH, Blum C, Yamamoto S. Crosstalk between alpha/beta T cells and gamma/delta T cells in vivo: activation of alpha/beta T-cell responses after gamma/delta T-cell modulation with the monoclonal antibody GL3. Proc Natl Acad Sci U S A. 1993; 90(20):9620-9624. (Clone-specific: Flow cytometry). View Reference
  4. King DP, Hyde DM, Jackson KA, et al. Cutting edge: protective response to pulmonary injury requires gamma delta T lymphocytes. J Immunol. 1999; 162(9):5033-5036. (Biology: Flow cytometry). View Reference
  5. Lefrancois L. Phenotypic complexity of intraepithelial lymphocytes of the small intestine. J Immunol. 1991; 147(6):1746-1751. (Biology: Flow cytometry). View Reference
  6. MacDonald HR, Schreyer M, Howe RC, Bron C. Selective expression of CD8 alpha (Ly-2) subunit on activated thymic gamma/delta cells. Eur J Immunol. 1990; 20(4):927-930. (Biology: Flow cytometry). View Reference
  7. Shinohara K, Ikarashi Y, Maruoka H, et al. Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains. Eur J Immunol. 1999; 29(6):1871-1878. (Biology: Flow cytometry). View Reference
  8. Skeen MJ, Ziegler HK. Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection. J Exp Med. 1993; 178(3):971-984. (Biology: Flow cytometry). View Reference
  9. Tamaki K, Yasaka N, Chang CH, et al. Identification and characterization of novel dermal Thy-1 antigen-bearing dendritic cells in murine skin. J Invest Dermatol. 1996; 106(3):571-575. (Biology: Flow cytometry). View Reference
  10. Tigelaar RE, Lewis JM, Bergstresser PR. TCR gamma/delta+ dendritic epidermal T cells as constituents of skin-associated lymphoid tissue. J Invest Dermatol. 1990; 94(6):58S-63S. (Biology: Flow cytometry). View Reference
  11. Vicari AP, Mocci S, Openshaw P, O'Garra A, Zlotnik A. Mouse gamma delta TCR+NK1.1+ thymocytes specifically produce interleukin-4, are major histocompatibility complex class I independent, and are developmentally related to alpha beta TCR+NK1.1+ thymocytes. Eur J Immunol. 1996; 26(7):1424-1429. (Biology: Flow cytometry). View Reference
View All (11) View Less
561996 Rev. 2

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.