The 11-5.2 monoclonal antibody specifically recognizes the α chain of the I-A[k] MHC class II alloantigen. It cross-reacts with cells from mice of the H-2[r] haplotype. mAb 11-5.2 recognizes the Ia.19 determinant, and it's reactivity is dependent upon the presence of Glu at position 75 of the Aα chain. Reactivity with other H-2 haplotypes (e.g., b, d, f, p, q, s) has not been observed.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.