The UP-D1 monoclonal antibody specifically binds to Immune receptor expressed on myeloid cells 1 (IREM-1). IREM-1 is encoded by CD300LF. It is also known as CD300 antigen-like family member F (CD300f), CMRF35-like molecule 1 (CLM-1/CLM1), NK inhibitory receptor (NKIR), or Immunoglobulin superfamily member 13 (IgSF13). IREM-1/CD300f is expressed on monocytes, dendritic cells, granulocytes, and mast cells. IREM-1 is a single-pass type I transmembrane glycoprotein that belongs to the CMRF family of the Ig gene superfamily. The long intracellular region of IREM-1 contains two classical immunoreceptor tyrosine-based inhibitory motifs (ITIM). Ligand-bound IREM-1 can transduce negative regulatory signals intracellularly by recruiting SHP1 or SHIP phosphatases that in turn dephosphorylate tyrosine residues within activating cell signaling molecules. CD300f may thus play a role in regulating inflammatory responses involving myeloid cells.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.