BUV737 Rat Anti-Mouse CD19
Two-color flow cytometric analysis of CD19 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 553066/561826) and either BUV737 Rat IgG2a, κ Isotype Control (Cat. No. 612760; Left Plot) or BD Horizon™ BUV 737 Rat Anti-Mouse CD19 antibody (Cat. No. 612781/612782; Right Plot) at 0.25 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The two-color fluorescence dot plot showing the correlated expression of CD19 (or Ig Isotype control staining) versus CD3e were derived from gated events with the forward and side light-scatter characteristic of viable (7-AAD-negative) splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown in this Technical Data Sheet are not lot specific.
BD Horizon™ BUV737 Rat Anti-Mouse CD19
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