BUV737 Hamster Anti-Mouse CD27
Two-color flow cytometric analysis of CD27 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 553066/561826) and either BD Horizon™ BV737 Hamster IgG1, κ Isotype Control (Cat. No. 564621; Left Plot) or BD Horizon BV737 Hamster Anti-Mouse CD27 antibody (Cat. No. 565307; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD27 (or Ig Isotype control staining) versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
BD Horizon™ BUV737 Hamster Anti-Mouse CD27
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