The 30B11.1 monoclonal antibody specifically binds to Delta-like1 (Dll1) which is also known as Delta1 and DL1. Dll1 is a 90-100 kDa type 1 transmembrane protein. It is a member of the Delta/Serrate/Jagged Family of Notch ligands. Notch ligands are classified by the presence of specific structural motifs including an N-terminal Delta-Serrate-LAG-2 (DSL) domain, EGF repeats, and the DOS domain (a specialized EGF repeat). The Notch family of transmembrane receptors and their ligands control cell-fate "decisions" during the development of many organs in a wide variety of animal species. Dll1 is expressed in the paraxial mesoderm and in some cells in the nervous system in early development. In the adult, Dll1 is expressed in a variety of tissues including at high levels in the heart and at various levels by cells within lymphoid tissues. Although Dll1 does not normally appear to play a role in T cell development, Dll1 and Notch interactions are essential for marginal zone B cell development and reportedly promote the differentiation of antibody producing cells.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).