The YLI-90 monoclonal antibody specifically reacts with the B6 alloantigen of Ly-49I, an inhibitory receptor which is expressed on subsets of natural killer (NK) cells, NK-1.1+ (or DX5+) T lymphocytes (NK-T cells), and on a population of memory CD8+ T lymphocytes in C57BL/6 mice, but not AKR/J, BALB/c, DBA/1, or SJL mice. The Ly-49 family of NK-cell receptors are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains (CRD). Ly-49I is closely related to Ly-49C[B6] and Ly49C[BALB], and these three alloantigens are recognized by mAb 5E6 (Cat. No. 553277). A Ly-49I[BALB] allele has not been found. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. Binding of Ly-49I-expressing transfectants to cell lines bearing MHC class I antigens of the b,d, k and s haplotypes was not detected. In contrast, Ly-49I-expressing transfectants were found to weakly bind lymphoblasts of the b, d, k, q, s, and v haplotypes and to strongly bind H-2[r] lymphoblasts. Ly-49I-bearing NK cells may mediate allogeneic and hybrid resistance to transplantation of H-2[d] bone marrow.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).