The AM64 monoclonal antibody specifically binds to CD130 which is also known as gp130 (Membrane glycoprotein 130), IL6ST (Interleukin-6 signal transducer), and IL6RB/IL-6Rβ (Interleukin-6 Receptor beta). CD130 is a 130 kDa type 1 membrane glycoprotein that belongs to the hemopoietic cytokine receptor superfamily. CD130 serves as a common signaling receptor for several cytokine receptor complexes including those that mediate the biological effects of IL-6, IL-11, IL-27, Cardiotrophin-1 (CT-1), Ciliary neurotrophic factor (CNTF), Leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD130 is widely expressed on a variety of cell types including T cells, activated B cells, monocytes, plasma cells, and endothelia. The AM64 antibody can be used in flow cytometry, immunoprecipitation, western blot, and immunohistochemistry on frozen sections.
The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.