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Biotin Rat Anti-Mouse CD249 (Ly-51)
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Product Details
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BD Pharmingen™
Bp-1/6C3; Ly51; Ly-51; Enpep; Glutamyl aminopeptidase; APA; EAP
Mouse (QC Testing)
Rat F344, also known as Fischer, CDF IgG2a, κ
C57L mouse Pre-B lymphhoma cell line L1-2
Flow cytometry (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  5. Please refer to for technical protocols.
553159 Rev. 10
Antibody Details
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The 6C3 monoclonal antibody specifically recognizes CD249 which is also known as, 6C3/BP-1, Enpep, or Ly-51. CD249 is a glycoprotein cell-surface differentiation antigen, which was originally identified on pre-B cell lymphomas (spontaneous and chemical- or retrovirus-transformed, in vitro and in vivo). CD249 is a homodimeric cell-surface glycoprotein with 140-kDa subunits that has been identified to possess aminopeptidase A (APA) activity. The same antigen is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoiesis, on thymic dendritic cells and cortical epithelial cells, and on a wide variety of mouse and rat tissues known to possess APA activity.  Subsets of normal bone marrow pre-B and B lymphocytes express low levels of CD249 which is rapidly up-regulated on the pre-B cells in the presence of IL-7. A role for the CD249 molecule in the IL-7-driven proliferation of B cell precursors has been postulated.  However, B-cell abnormalities were not detected in CD249/Ly-51-deficient mice. Mature B lymphocytes, thymocytes, peripheral T lymphocytes, erythroid cells, and myeloid cells (with the exception of thymic dendritic cells) do not express CD249. The 6C3 antibody can be used to identify cortical epithelium in frozen sections of thymuses from normal, SCID, and TCR-transgenic mice.  It is possible that the low level of CD249 antigen detected, by flow cytometry, on some thymocytes may be passively adsorbed from adjacent epithelial cells during preparation of the cell suspensions.

553159 Rev. 10
Format Details
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Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
553159 Rev.10
Citations & References
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Development References (19)

  1. Adkins B, Tidmarsh GF, Weissman IL. Normal thymic cortical epithelial cells developmentally regulate the expression of a B-lineage transformation-associated antigen. Immunity. 1988; 27(3):180-186. (Biology). View Reference
  2. Goverman J, Brabb T, Huseby ES, Farr AG. TCR signaling regulates thymic organization: lessons from TCR-transgenic mice. Immunol Today. 1997; 18(5):204-208. (Biology). View Reference
  3. Hardy RR, Carmack CE, Shinton SA, Kemp JD, Hayakawa K. Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow. J Exp Med. 1991; 173(5):1213-1225. (Biology). View Reference
  4. Li L, Wu Q, Wang J, Bucy RP, Cooper MD. Widespread tissue distribution of aminopeptidase A, an evolutionarily conserved ectoenzyme recognized by the BP-1 antibody. Tissue Antigens. 1993; 42(5):488-496. (Biology). View Reference
  5. Lin Q, Taniuchi I, Kitamura D. T and B cell development in BP-1/6C3/aminopeptidase A-deficient mice. Int Immunol. 1998; 160(10):4681-4687. (Biology). View Reference
  6. Morse HC 3rd, Tidmarsh GF, Holmes KL. Expression of the 6C3 antigen on murine hematopoietic neoplasms. Association with expression of abl, ras, fes, src, erbB, and Cas NS-1 oncogenes but not with myc. J Exp Med. 1987; 165(3):920-925. (Biology). View Reference
  7. Pillemer E, Whitlock C, Weissman IL. Transformation-associated proteins in murine B-cell lymphomas that are distinct from Abelson virus gene products. Proc Natl Acad Sci U S A. 1984; 81(14):4434-4438. (Immunogen). View Reference
  8. Ramakrishnan L, Wu Q, Yue A, Cooper MD, Rosenberg N. BP-1/6C3 expression defines a differentiation stage of transformed pre-B cells and is not related to malignant potential. J Immunol. 1990; 145(5):1603-1608. (Biology). View Reference
  9. Sherwood PJ, Weissman IL. The growth factor IL-7 induces expression of a transformation-associated antigen in normal pre-B cells. Int Immunol. 1990; 2(5):399-406. (Biology). View Reference
  10. Surh CD, Ernst B, Sprent J. Growth of epithelial cells in the thymic medulla is under the control of mature T cells. J Exp Med. 1992; 172(2):611-616. (Biology). View Reference
  11. Tidmarsh GF, Dailey MO, Whitlock CA, Pillemer E, Weissman IL. Transformed lymphocytes from Abelson-diseased mice express levels of a B lineage transformation-associated antigen elevated from that found on normal lymphocytes. J Exp Med. 1985; 162(5):1421-1434. (Biology). View Reference
  12. Vremec D, Shortman K. Dendritic cell subtypes in mouse lymphoid organs: cross-correlation of surface markers, changes with incubation, and differences among thymus, spleen, and lymph nodes. J Immunol. 1997; 159(2):565-573. (Biology). View Reference
  13. Wang J, Cooper MD. Histidine residue in the zinc-binding motif of aminopeptidase A is critical for enzymatic activity. Proc Natl Acad Sci U S A. 1990; 90(4):1222-1226. (Biology). View Reference
  14. Welch PA, Burrows PD, Namen A, Gillis S, Cooper MD. Bone marrow stromal cells and interleukin-7 induce coordinate expression of the BP-1/6C3 antigen and pre-B cell growth. Int Immunol. 1990; 2(8):697-705. (Biology). View Reference
  15. Welch PA. Regulation of B cell precursor proliferation by aminopeptidase A. Int Immunol. 1995; 7(5):737-746. (Biology). View Reference
  16. Wu L, Vremec D, Ardavin C, et al. Mouse thymus dendritic cells: kinetics of development and changes in surface markers during maturation. Eur J Immunol. 1995; 25(2):418-425. (Biology). View Reference
  17. Wu Q, Lahti JM, Air GM, Burrows PD, Cooper MD. Molecular cloning of the murine BP-1/6C3 antigen: a member of the zinc-dependent metallopeptidase family. Proc Natl Acad Sci U S A. 1990; 87(3):993-997. (Biology). View Reference
  18. Wu Q, Li L, Cooper MD, Pierres M, Gorvel JP. Aminopeptidase A activity of the murine B-lymphocyte differentiation antigen BP-1/6C3. Proc Natl Acad Sci U S A. 1991; 88(2):676-680. (Biology). View Reference
  19. Wu Q, Tidmarsh GF, Welch PA, Pierce JH, Weissman IL, Cooper MD. The early B lineage antigen BP-1 and the transformation-associated antigen 6C3 are on the same molecule. J Immunol. 1989; 143(10):3303-3308. (Biology). View Reference
View All (19) View Less
553159 Rev. 10

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.