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Biotin Rat Anti-Mouse CD115 (CSF-1R)
Biotin Rat Anti-Mouse CD115 (CSF-1R)
Multicolor flow cytometric analysis of CD115 (CSF-1R) expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were stained with BD Horizon™ BV421 Rat Anti-Mouse CD11b antibody (Cat. No. 562605) and either Biotin Rat IgG1, κ Isotype Control (Cat. No. 553923; Left Plot) or Biotin Rat Anti-Mouse CD115 (CSF-1R) antibody (Cat. No. 567598; Right Plot) at 0.25 µg/test, followed by PE Streptavidin (Cat. No. 554061). A bivariate pseudocolor density plot showing the correlated expression of CD115 (CSF-1R) [or Ig Isotype control staining] versus CD11b was derived from gated events with the forward and side-light scattering characteristics of intact bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Multicolor flow cytometric analysis of CD115 (CSF-1R) expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were stained with BD Horizon™ BV421 Rat Anti-Mouse CD11b antibody (Cat. No. 562605) and either Biotin Rat IgG1, κ Isotype Control (Cat. No. 553923; Left Plot) or Biotin Rat Anti-Mouse CD115 (CSF-1R) antibody (Cat. No. 567598; Right Plot) at 0.25 µg/test, followed by PE Streptavidin (Cat. No. 554061). A bivariate pseudocolor density plot showing the correlated expression of CD115 (CSF-1R) [or Ig Isotype control staining] versus CD11b was derived from gated events with the forward and side-light scattering characteristics of intact bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
M-CSFR; M-CSF-R; CSF-1 Receptor; CSF-1R; Csf1r; Csfmr; Fms; c-Fms; Fim-2
Mouse (QC Testing)
Rat IgG1, κ
Mouse CD115 Recombinant Protein
Flow cytometry (Routinely Tested)
0.5 mg/ml
12978
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
567598 Rev. 1
Antibody Details
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T38-320

The T38-320 monoclonal antibody specifically binds to CD115 which is also known as Colony stimulating factor 1 Receptor (CSF-1R) or Macrophage colony-stimulating factor 1 receptor (M-CSFR). This type I transmembrane glycoprotein is a receptor tyrosine kinase (RTK) that belongs to the Ig superfamily. It is expressed on a variety of cells including those committed to the mononuclear phagocyte lineage, such as, monocytes, macrophages, and osteoclasts. CSF-1 binds to and signals through CSF-1R homodimers which undergo tyrosine autophosphorylation and transduce downstream signaling pathways resulting in cytoskeletal reorganization and gene expression. CSF-1R activation stimulates the proliferation, differentiation, and survival of cells within the mononuclear phagocyte system.  Interleukin-34 (IL-34) is another ligand for CD115 that can induce similar, as well as, some different biological responses by CD115-positive target cells.

567598 Rev. 1
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
567598 Rev.1
Citations & References
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Development References (7)

  1. Breslin WL, Strohacker K, Carpenter KC, Haviland DL, McFarlin BK. Mouse blood monocytes: standardizing their identification and analysis using CD115. J Immunol Methods. 2013; 390(1-2):1-8. (Biology). View Reference
  2. De Schepper S, Verheijden S, Aguilera-Lizarraga J, et al. Self-Maintaining Gut Macrophages Are Essential for Intestinal Homeostasis.. Cell. 2018; 175(2):400-415.e13. (Clone-specific: Flow cytometry). View Reference
  3. Fend L, Accart N, Kintz J et al. Therapeutic effects of anti-CD115 monoclonal antibody in mouse cancer models through dual inhibition of tumor-associated macrophages and osteoclasts. PLoS ONE. 2013; 8(9):e73310. (Biology). View Reference
  4. Huang B, Pan PY, Li Q et al. Gr-1+CD115+ immature myeloid suppressor cells mediate the development of tumor-induced T regulatory cells and T-cell anergy in tumor-bearing host. Cancer Res. 2006; 15(66):1123-1131. (Biology). View Reference
  5. Muench DE, Olsson A, Ferchen K, et al. Mouse models of neutropenia reveal progenitor-stage-specific defects. Nature. 2020; 582(7810):109-114. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
  6. Rothwell VM, Rohrschneider LR. Murine c-fms cDNA: cloning, sequence analysis and retroviral expression. Oncogene Res. 1987; 1(4):311-324. (Biology). View Reference
  7. Zhang Z, Dong L, Jia A, et al. Glucocorticoids Promote the Onset of Acute Experimental Colitis and Cancer by Upregulating mTOR Signaling in Intestinal Epithelial Cells.. Cancers (Basel). 2020; 12(4):E945. (Clone-specific: Flow cytometry). View Reference
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567598 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.