Biotin Mouse Anti-Rat IgG1/2a

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BD Pharmingen™ Biotin Mouse Anti-Rat IgG1/2a

Name: Biotin Mouse Anti-Rat IgG1/2a
Brand: BD Pharmingen™
SKU: 550325

Clone G28-5

(RUO)

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Product Details

Brand: BD Pharmingen™

Alternative Name: IgG1/IgG2a; Ig gamma-1/2a; Immunoglobulin gamma 1/2a

Reactivity: Rat (QC Testing), Mouse (Tested in Development)

Isotype: Mouse IgG2a

Immunogen: Pooled Rat IgG

Application: ELISA (Routinely Tested), Flow cytometry, Immunohistochemistry-frozen, Immunohistochemistry-paraffin (Tested During Development)

Concentration: 250 µg/ml

Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

Immunohistochemistry: Investigators are highly encouraged to titrate the antibody for each individual application. A dilution range of (1:12.5 - 1:100) is suggested.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

DAB Substrate Kit RUO

Size 500 Tests Cat No. 550880

Antibody Diluent for IHC RUO

Size 125 mL Cat No. 559148

Streptavidin HRP RUO

Size 50 mL Cat No. 550946

550325 Rev. 8

Antibody Details

G28-5

The G28-5 monoclonal antibody reacts specifically with rat IgG1 and IgG2a. It does not react with other Ig isotypes. A suspension of pooled rat IgG1 was used as the source of immunogen.

550325 Rev. 8

Format Details

Biotin

Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.

Format: Biotin

550325 Rev.8

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.