Alexa Fluor® 700 Rat anti-Mouse Ly-6G and Ly-6C
Two-parameter analysis of the expression of Ly-6G and/or Ly-6C on bone-marrow myeloid cells. C57BL/6 bone-marrow leukocytes were stained with either Alexa Fluor® 700-conjugated Rat IgG2b, k isotype control mAb A95-1 (Cat. no. 557964, Panel A) or Alexa Fluor® 700-conjugated mAb RB6-8C5 (Panel B) in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. no. 553141/553142). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) shows little expression of Ly-6G/Ly-6C, while most of the leukocytes with moderate-to-high SSC (myeloid cells) are Ly-6G/Ly-6C-positive. Flow cytometry was performed on a BD™ LSR II flow cytometry system.
BD Pharmingen™ Alexa Fluor® 700 Rat anti-Mouse Ly-6G and Ly-6C
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