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Alexa Fluor® 647 Mouse Anti-TCF-7/TCF-1
Alexa Fluor® 647 Mouse Anti-TCF-7/TCF-1
Two-color flow cytometric analysis of TCF-7/TCF-1 expression by human lymphocytes or mouse splenocytes.      Upper Plots - Human peripheral blood mononuclear cells (PBMC) were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were then stained with PE Mouse Anti-Human CD4 antibody (Cat. No. 555347/561843/561844) and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; Left Plot) or Alexa Fluor® 647 Mouse Anti-TCF-7/TCF-1 antibody (Cat. No. 566693; Right Plot) at 0.06 μg/test.      Lower Plots - Mouse splenic leucocytes were similarly fixed and permeabilized. The cells were then stained with PE Rat Anti-Mouse CD4 antibody (Cat. No. 553048/553049/561837) and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Left Plot) or Alexa Fluor® 647 Mouse Anti-TCF-7/TCF-1 antibody (Right Plot) at 0.06 μg/test.      Bivariate pseudocolor density plots showing the correlated expression of CD4 versus TCF-7/TCF-1 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Two-color flow cytometric analysis of TCF-7/TCF-1 expression by human lymphocytes or mouse splenocytes.      Upper Plots - Human peripheral blood mononuclear cells (PBMC) were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were then stained with PE Mouse Anti-Human CD4 antibody (Cat. No. 555347/561843/561844) and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; Left Plot) or Alexa Fluor® 647 Mouse Anti-TCF-7/TCF-1 antibody (Cat. No. 566693; Right Plot) at 0.06 μg/test.      Lower Plots - Mouse splenic leucocytes were similarly fixed and permeabilized. The cells were then stained with PE Rat Anti-Mouse CD4 antibody (Cat. No. 553048/553049/561837) and either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Left Plot) or Alexa Fluor® 647 Mouse Anti-TCF-7/TCF-1 antibody (Right Plot) at 0.06 μg/test.      Bivariate pseudocolor density plots showing the correlated expression of CD4 versus TCF-7/TCF-1 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
TCF7; Transcription factor 7; TCF-1; TCF1; T-cell factor 1
Mouse (QC Testing), Human (Tested in Development)
Mouse IgG1, κ
Mouse TCF-7 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2869823
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
  9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566693 Rev. 3
Antibody Details
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S33-966

The S33-966 monoclonal antibody specifically binds to TCF-7 (Transcription factor 7) which is also known as TCF-1 (T-cell factor 1). TCF-7 is a member of the High Mobility Group (HMG) DNA binding protein family of transcription factors. TCF-7 is expressed in thymocytes, T lymphocytes and proliferating epithelial cells. TCF-7 is expressed in the earliest T cell precursors and serves as a critical transcriptional regulator that is involved in the differentiation of thymocytes to become mature T lymphocytes. TCF-7 is necessary for the survival of immature CD4+CD8+ thymocytes. TCF-7 can also promote the differentiation of mature peripheral T cells to become Th2-like cells. TCF-7 may also function as a transcriptional repressor of certain target genes including CTNNB1 and TCF7L2. The S33-966 antibody crossreacts with human TCF-7.

566693 Rev. 3
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
566693 Rev.3
Citations & References
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Development References (5)

  1. Chen Z, Ji Z, Ngiow SF, et al. TCF-1-Centered Transcriptional Network Drives an Effector versus Exhausted CD8 T Cell-Fate Decision. Immunity. 2019; 51(5):840-855. (Clone-specific: Flow cytometry). View Reference
  2. Germar K, Dose M, Konstantinou T, et al. T-cell factor 1 is a gatekeeper for T-cell specification in response to Notch signaling. Proc Natl Acad Sci U S A. 2011; 108(50):20060-20065. (Biology). View Reference
  3. Johnson JL, Georgakilas G, Petrovic J, et al. Lineage-Determining Transcription Factor TCF-1 Initiates the Epigenetic Identity of T Cells.. Immunity. 2018; 48(2):243-257.e10. (Clone-specific: Flow cytometry). View Reference
  4. Weber BN, Chi AW, Chavez A, et al. A critical role for TCF-1 in T-lineage specification and differentiation. Nature. 2011; 476(7358):63-68. (Biology). View Reference
  5. Yu Q, Sharma A, Oh SY, et al. T cell factor 1 initiates the T helper type 2 fate by inducing the transcription factor GATA-3 and repressing interferon-γ. Nat Immunol. 2009; 10(9):992-999. (Biology). View Reference
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566693 Rev. 3

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.