Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
The E23.1 monoclonal antibody specifically recognizes Cyclin A. Several classes of cyclins (A-E) have been characterized which act as regulatory subunits for Cyclin-dependent kinases (Cdks). These Cyclin-Cdk holoenzymes are essential for proper control of cell cycle progression. They phosphorylate and regulate a variety of substrates whose activity is required for cell cycle transitions. The temporal expression of cyclins is tightly regulated throughout the cell cycle by synthesis and degradation. Such regulation plays a critical role in controlling the enzymatic activity of the Cdks. Cyclin A, one of the mitotic cyclins, activates Cdk2 near the start of S phase and is necessary for the initiation of DNA replication. In mammalian somatic cells, Cyclin A is required during S phase and passage through G2. The D and E type cyclins regulate passage through G1, while Cyclin B is a critical regulator of mitosis. It has been shown in several species that mutation or disruption of normal Cyclin A expression causes cells to arrest at G2. Cyclin A binds both the Cdk1 and Cdk2 kinases and may also have a role in mitotic dependence on S phase completion. Cyclin A can serve as a marker for actively proliferating cells and in S phase.
Development References (5)
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Adamczewski JP, Gannon JV, Hunt T. Simian virus 40 large T antigen associates with cyclin A and p33cdk2.. J Virol. 1993; 67(11):6551-7. (Immunogen: Immunoprecipitation, Radioimmunoassay). View Reference
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Darzynkiewicz Z, Gong J, Juan G, Ardelt B, Traganos F. Cytometry of cyclin proteins.. Cytometry. 1996; 25(1):1-13. (Methodology). View Reference
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Geley S, Kramer E, Gieffers C, Gannon J, Peters JM, Hunt T. Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint.. J Cell Biol. 2001; 153(1):137-48. (Immunogen: Immunofluorescence, Western blot). View Reference
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Hégarat N, Crncec A, Suarez Peredo Rodriguez MF, et al. Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B.. EMBO J. 2020; 39(11):e104419. (Biology). View Reference
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Mateo F, Vidal-Laliena M, Canela N, et al. Degradation of cyclin A is regulated by acetylation.. Oncogene. 2009; 28(29):2654-66. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.