Skip to main content Skip to navigation
Alexa Fluor® 647 Hamster Anti-Rat CD29
Alexa Fluor® 647 Hamster Anti-Rat CD29

Flow cytometric analysis of CD29 expressed on rat splenocytes.  Lewis rat splenocytes were stained with either Alexa Fluor® 647 Armenian Hamster IgM, λ1 Isotype Control (Cat. No. 562110, dashed line histogram) or an Alexa Fluor® 647 Hamster Anti-Rat CD29 antibody (Cat. No. 562153, solid line histogram). Flow cytometric fluorescence histograms were derived from gated events based on forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Flow cytometric analysis of CD29 expressed on rat splenocytes.  Lewis rat splenocytes were stained with either Alexa Fluor® 647 Armenian Hamster IgM, λ1 Isotype Control (Cat. No. 562110, dashed line histogram) or an Alexa Fluor® 647 Hamster Anti-Rat CD29 antibody (Cat. No. 562153, solid line histogram). Flow cytometric fluorescence histograms were derived from gated events based on forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
Itgb1; Integrin β1 chain; Integrin beta-1; VLA-4 subunit beta
Rat (QC Testing)
Armenian Hamster IgM, κ
Rat glomerular epithelial cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_10896298
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
562153 Rev. 1
Antibody Details
Down Arrow Up Arrow
Ha2/5

The Ha2/5 monoclonal antibody specifically binds to the 130 kDa integrin β1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αV (CD51), it forms αVβ1 integrin. As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, and epithelia. The Ha2/5 hamster anti-rat CD29 monoclonal antibody cross-reacts with mouse thymocytes, splenocytes, and peripheral lymph node leukocytes. The Ha2/5 antibody blocks in vitro adhesion of CD29-expressing cells to collagen.

562153 Rev. 1
Format Details
Down Arrow Up Arrow
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
562153 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (2)

  1. Mendrick DL, Kelly DM. Temporal expression of VLA-2 and modulation of its ligand specificity by rat glomerular epithelial cells in vitro. Lab Invest. 1993; 69(6):690-702. (Immunogen). View Reference
  2. Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Biology). View Reference
562153 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.