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      Alexa Fluor™ 488 Mouse Anti-Human CD98
      Alexa Fluor™ 488 Mouse Anti-Human CD98

      Flow cytometric analysis of CD98 expression on Human peripheral blood leucocyte populations.  Fresh whole blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/564220) and then stained with  Alexa Fluor™ 488 Mouse IgG1, κ Isotype Control (Cat. No. 565572; Left Plot) or Alexa Fluor™ 488 Mouse Anti-Human CD98 antibody (Cat. No. 569024/569025; Right Plot). The bivariate pseudocolor density plot showing the correlated expression of CD98 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

      Alexa Fluor™ 488 Mouse Anti-Human CD98

      Flow cytometric analysis of CD98 expression on Human peripheral blood leucocyte populations.  Fresh whole blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/564220) and then stained with  Alexa Fluor™ 488 Mouse IgG1, κ Isotype Control (Cat. No. 565572; Left Plot) or Alexa Fluor™ 488 Mouse Anti-Human CD98 antibody (Cat. No. 569024/569025; Right Plot). The bivariate pseudocolor density plot showing the correlated expression of CD98 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

      Product Details
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      BD Pharmingen™
      SLC3A2; CD98; CD98 Heavy Chain; CD98HC; 4F2; 4F2hc
      Human (QC Testing)
      Mouse IgG1, κ
      Human Raji Cell Line
      Flow cytometry (Routinely Tested)
      5 µl/test
      VI BP 409, NL N-L017
      AB_3684718
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. An isotype control should be used at the same concentration as the antibody of interest.
      8. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
      9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      10. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      11. For U.S. patents that may apply, see bd.com/patents.
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      569024 Rev. 1
      Antibody Details
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      MEM-108

      The MEM-108 monoclonal antibody specifically recognizes the Human CD98 Heavy Chain (CD98HC or CD98) which is also known as FRP-1, or the 4F2 heavy chain antigen (4F2hc or 4F2). CD98 is a heterodimer that is comprised of an ~80-85 type II transmembrane glycoprotein heavy chain that is disulfide linked to one of at least six different ~40-45 kDa CD98 light chains. The CD98HC is comprised of a large heavily glycosylated extracellular domain followed by a short transmembrane segment and cytoplasmic tail. The latter CD98HC segments can associate with β1 integrins and regulate their signaling functions whereas the light chain functions in amino acid transport. CD98 is variably expressed on hematopoietic and non-hematopoietic cells including T cells, B cells, NK cells, and monocytes. CD98 expression is highly upregulated on activated and proliferating cells including tumor cells.

      569024 Rev. 1
      Format Details
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      Alexa Fluor™ 488
      Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 488
      Blue 488 nm
      494 nm
      517 nm
      569024 Rev.1
      Citations & References
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      View product citations for antibody "569024" on CiteAb

      Development References (5)

      1. Cantor J, Browne CD, Ruppert R, et al. CD98hc facilitates B cell proliferation and adaptive humoral immunity.. Nat Immunol. 2009; 10(4):412-9. (Biology). View Reference
      2. Diaz LA, Antony PA, Endres J, and Fox DA. CD98 Workshop Panel Report. In: Kishimoto T, ed. Leukocyte Typing VI. New York: Garland Publishing; 1997:531-534.
      3. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
      4. Woodhead VE, Stonehouse TJ, Binks MH, et al. Novel molecular mechanisms of dendritic cell-induced T cell activation.. Int Immunol. 2000; 12(7):1051-61. (Clone-specific: Flow cytometry). View Reference
      5. Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
      View All (5) View Less
      569024 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.