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      V450 Mouse IgG2b, κ Isotype Control
      V450 Mouse IgG2b, κ Isotype Control
      Analysis of BD Horizon™ V450 Mouse IgG2b, κ Isotype Control on human lymphocytes. Whole blood was stained with BD Horizon™ V450 Mouse IgG2b, κ Isotype Control (solid line) or left unstimulated (dotted line). Flow cytometry was performed on a BD LSR™ II flow cytometry system.
      V450 Mouse IgG2b, κ Isotype Control
      Analysis of BD Horizon™ V450 Mouse IgG2b, κ Isotype Control on human lymphocytes. Whole blood was stained with BD Horizon™ V450 Mouse IgG2b, κ Isotype Control (solid line) or left unstimulated (dotted line). Flow cytometry was performed on a BD LSR™ II flow cytometry system.
      Product Details
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      BD Horizon™
      Isotype Control (anti-Dansyl); Anti-Dansyl; Anti-DNS
      Human (QC Testing)
      Mouse C.SW IgG2b, κ
      Flow cytometry, Isotype control (Routinely Tested)
      0.2 mg/ml
      AB_1645679
      Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Product Notices

      1. An isotype control should be used at the same concentration as the antibody of interest.
      2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      4. BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
      5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
      6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      560374 Rev. 1
      Antibody Details
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      27-35

      This mouse IgG2b, κ isotype control is a monoclonal antibody, clone 27-35, that is specific for the dansyl (5-[dimethylamino] naphthalene-1-sulfonyl) hapten. The dansyl (DNS) hapten is not expressed on human cells or human cell lines. The 27-35 immunoglobulin was selected as an isotype control following testing that demonstrated low background staining on a variety of mouse and human tissues.

      The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

      560374 Rev. 1
      Format Details
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      V450
      BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      V450
      Violet 405 nm
      405 nm
      450 nm
      560374 Rev.1
      Citations & References
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      Development References (3)

      1. BD Biosciences. Techniques for Immune Function Analysis, Application Handbook 1st Edition. 2003. Available: http://www.bdbiosciences.com/pdfs/manuals/02-8100055-21A1rr.pdf 2007, Jan. 25.
      2. Paliard P, Vitrey D, Fournier G, Belhadjali J, Patricot L, Berger F. Perhexiline maleate-induced hepatitis.. Digestion. 1978; 17(5):419-27. (Clone-specific). View Reference
      3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). View Reference
      560374 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.