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PE Mouse Anti-Human IL-4
Product Details
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BD FastImmune™
Human
Mouse IgG1
Recombinant human IL-4
Intracellular staining (flow cytometry)
1.25 μg/mL
20 μL
3565
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340451 Rev. 1
Antibody Details
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3010.211

The Anti-Hu–IL-4 antibody, clone 3010.211, is derived from the hybridization of Sp2/0 mouse cells with spleen cells from BALB/c mice immunized with recombinant human IL-4.

The Anti-Human Interleukin-4 (Anti-Hu–IL-4) antibody recognizes a 15- to 19-kilodalton (kDa) glycoprotein.

340451 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
340451 Rev.1
Citations & References
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Development References (22)

  1. Aversa G, Punnonen J, Cocks B G, et al. An Interleukin 4 (IL-4) mutant protein inhibits both IL-3 or IL-13-induced human immunoglobulin G4 (IgG4) and IgE synthesis and B cell proliferation: support for a common component shared by IL-4 and IL-13 receptors. J Exp Med. 1993; 178:2213-2218. (Biology).
  2. Bradding P, Feather IH, Howarth PH, et al. Interleukin 4 is localized to and released by human mast cells. J Exp Med. 1992; 176:1381-1386. (Biology).
  3. Carding SR, Hayday AC, Bottomly K. Cytokines in T-cell development. Immunol Today. 1991; 12:239-245. (Biology).
  4. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  5. Clerici M, Hakim FT, Venzon DJ, et al. Changes in interleukin-2 and interleukin-4 production in asymptomatic, human immunodeficiency virus-seropositive individuals. J Clin Invest. 1993; 91:759-765. (Biology).
  6. Clerici M, Shearer GM. A TH1 → TH2 switch is a critical step in the etiology of HIV infection. Today. 1993; 14:107-110. (Biology).
  7. Clinical and Laboratory Standards Institute. 2005. (Biology).
  8. ElGhazali GEB, Paulie S, Andersson G, et al. Number of interleukin-4– and interferon- γ –secreting human T cells reactive with tetanus toxoid and the mycobacterial antigen PPD or phytohemagglutindistinct response profiles depending on the type of antigen used for activation. Eur J Immunol. 1993; 23:2740-2745. (Biology).
  9. Elliott MJ, Gamble JR, Park LS, Vadas MA, Lopez AF. Inhibition of human monocyte adhesion by interleukin-4. Blood. 1991; 77:2739-2745. (Biology).
  10. Elson LH, Nutman TB, Metcalfe DD, Prussin C. Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4+ CD27- lymphocyte subpopulation. J Immunol. 1995; 154:4294-4301. (Biology).
  11. Graziosi C, Pantaleo G, Gantt KR, et al. Lack of evidence for the dichotomy of T H 1 and T H 2 predominance in HIV-infected individuals. Science. 1994; 265:248-252. (Biology).
  12. Hyjek E, Lischner HW, Hyslop T, et al. Cytokine patterns during progression to AIDS in children with perinatal HIV infection. J Immunol. 1995; 155:4060-4071. (Biology).
  13. Idzerda RL, March CJ, Mosley B, et al. Human interleukin 4 receptor confers biological responsiveness and defines a novel receptor superfamily. J Exp Med. 1990; 171(3):861-873. (Biology). View Reference
  14. Keegan AD, Nelms K, Wang L-M, Pierce JH, Paul WE. Interleukin 4 receptor: signaling mechanisms. Immunol Today. 1994; 15:423-432. (Biology).
  15. Maggi E, Mazzetti M, Ravina A, et al. Ability of HIV to promote a TH1 to TH0 shift and to replicate preferentially in TH2 and TH 0 cells. Science. 1994; 265:244-248. (Biology).
  16. Nagler A, Lanier LL, Phillips JH. The effects of IL-4 on human natural killer cells: A potent regulator of IL-2 activation and proliferation. J Immunol. 1988; 141:2349-2351. (Biology).
  17. Openshaw P, Murphy EE, Hosken NA, et al. Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations. J Exp Med. 1995; 182(5):1357-1367. (Biology). View Reference
  18. Paul WE. Interleukin-4: a prototypic immunoregulatory lymphokine. Blood. 1991; 77(9):1859-1870. (Biology). View Reference
  19. Picker LJ, Singh MK, Zdraveski Z, et al. Direct demonstration of cytokine synthesis heterogeneity among human memory/effector T cells by flow cytometry. Blood. 1995; 86:1408-1419. (Biology).
  20. Powrie F, Coffman RL. Cytokine regulation of T-cell function: potential for therapeutic intervention. Immunol Today. 1993; 14:270-274. (Biology).
  21. Romagnani S, Del Prete G, Maggi E, et al. Human TH1 and TH2 subsets. Int Arch Allergy Immunol. 1992; 99:242-245. (Biology).
  22. Street NE, Mosmann TR. Functional diversity of T lymphocytes due to secretion of different cytokine patterns. FASEB J. 1991; 5:171-176. (Biology).
View All (22) View Less
340451 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not For Use in Diagnostic Procedures.

RUO/GMP—For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

23-22943-00