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PE Mouse Anti-Human IL-1α
Product Details
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BD FastImmune™
Human
Mouse BALB/c IgG1, κ
Human
Flow cytometry
12.5 μg/mL
20 μL
3552
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340514 Rev. 2
Antibody Details
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AS5

The Anti-Hu–IL-1α antibody, clone AS5, is derived from fusion of P3X63Ag8 myeloma cells with splenocytes from BALB/c mice immunized with recombinant human IL-1α.

The Anti-Human Interleukin-1α (Anti-Hu–IL-1α) antibody recognizes a 13- to 18-kilodalton (kDa) polypeptide.

340514 Rev. 2
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
340514 Rev.2
Citations & References
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Development References (14)

  1. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  2. Clinical and Laboratory Standards Institute. 2005. (Biology).
  3. Conlon PJ, Grabstein KH, Alpert A, et al. Localization of human mononuclear cell interleukin 1. J Immunol. 1987; 139:98-102. (Biology).
  4. Dinarello CA. Biology of interleukin 1. FASEB J. 1988; 2:108-115. (Biology).
  5. Dinarello CA. Interleukin-1 and interleukin-1 antagonism. Blood. 1991; 77(8):1627-1652. (Biology). View Reference
  6. Fini ME, Strissel KJ, Girard MT, Mays JW, Rinehart WB. Interleukin 1αmediates collegenase synthesis stimulated by Phorbol 12-Myristate 13-Acetate. J Bio Chem. 1994; 269:11291-11298. (Biology).
  7. Giri JG, Lomedico PT, Mizel SB. Studies on the synthesis and secretion of interleukin 1. I. A 33,000 molecular weight precursor for interleukin 1. J Immunol. 1985; 134(1):343-349. (Biology). View Reference
  8. Mantovani A, Dejana E. Cytokines as communication signals between leukocytes and endothelial cells.. Immunol Today. 1989; 10(11):370-5. (Biology). View Reference
  9. Matsushima K, Yodoi J, Tagaya Y, Oppenheim JJ. Downregulation of interleukin-1 receptor expression by IL-1 and fate of internalized125 I-labeled IL-1 β in a human large granular lymphocyte cell line. J Immunol. 1986; 137:3183-3188. (Biology).
  10. Mizel SB. The interleukins. FASEB J. 1989; 32379-2388. (Biology).
  11. Saklatvala J. Tumour necrosis factor α stimulates resorption and inhibits synthesis of proteoglycan in cartilage. Science. 1986; 322:547-549. (Biology).
  12. Slack J, McMahan CJ, Waugh S. et al. Independent binding of interleukin-1 α and interleukin-1 β to type I and type II interleukin 1 receptors. J Bio Chem. 1993; 268:2513-2524. (Biology).
  13. Van Zee KJ, DeForge LE, Fischer E, et al. IL-8 in septic shock, endotoxemia and after IL-1 administration. J Immunol. 1991; 146:3478-3482. (Biology).
  14. West-Mays JA, Strissel KJ, Sadow PM, Fini ME. Competence for collegenase gene expression by tissue fibroblasts requires activation of an interleukin 1α autocrine loop. Prod Natl Acad Sci. 1995; 92:6768-6772. (Biology).
View All (14) View Less
340514 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not For Use in Diagnostic Procedures.

RUO/GMP—For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

23-22943-00