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PE Mouse Anti-Human CD154
Product Details
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CD40LG; CD40 ligand; CD40L; gp39; TNFSF5; T-BAM; TRAP
Mouse BALB/c IgG1, κ
sgp39-CD8 and Activated Human PBL
Flow cytometry
12.5 μg/mL
20 μL
Phosphate buffered saline with gelatin and 0.1% sodium azide.

Preparation And Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340477 Rev. 1
Antibody Details
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CD154, clone 89-76, is derived from hybridization of mouse NS-1 cells with spleen cells from BALB/c mice immunized with sgp39-CD8 and activated human peripheral blood lymphocytes (PBLs).

CD154 (CD40 ligand or CD40L) recognizes a 39-kilodalton (kDa) antigen that is a member of the tumor necrosis factor (TNF) receptor family. CD154 is transiently expressed on activated CD4 T lymphocytes. The expression of CD154 by activated T-helper cells triggers B-cell cycling through binding to CD40. This co-receptor interaction is required for B-lymphocyte maturation, response to T-dependent ligands, and isotype switching. T-lymphocyte proliferation and generation of cytotoxic T lymphocytes (CTLs) is associated with the presence of CD154. Defects in the CD154 gene are associated with the X-linked hyper IgM syndrome.

340477 Rev. 1
Format Details
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R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
340477 Rev.1
Citations & References
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View product citations for antibody "340477" on CiteAb

Development References (23)

  1. Armitage RJ, Fanslow WC, Strockbine L, et al. Molecular and biological characterization of a murine ligand for CD40.. Nature. 1992; 357(6373):80-2. (Biology). View Reference
  2. Aruffo A, Farrington M, Hollenbaugh D, et al. The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome.. Cell. 1993; 72(2):291-300. (Biology). View Reference
  3. Ashokkumar C, Talukdar A, Sun Q, et al. Allospecific CD154+ T cells associate with rejection risk after pediatric liver transplantation.. Am J Transplant. 2009; 9(1):179-91. (Biology). View Reference
  4. Axcrona K, Gray D, Leanderson T. Regulation of B cell growth and differentiation via CD21 and CD40. Eur J Immunol. 1996; 26(9):2203-2207. (Biology). View Reference
  5. Bour-Jordan H, Bluestone JA. Regulating the regulators: costimulatory signals control the homeostasis and function of regulatory T cells.. Immunol Rev. 2009; 229(1):41-66. (Biology). View Reference
  6. Carbone A, Gloghini A, Gruss HJ, Pinto A. CD40 ligand is constitutively expressed in a subset of T cell lymphomas and on the microenvironmental reactive T cells of follicular lymphomas and Hodgkin's disease.. Am J Pathol. 1995; 147(4):912-22. (Biology). View Reference
  7. Cayabyab M, Phillips JH, Lanier LL. CD40 preferentially costimulates activation of CD4+ T lymphocytes.. J Immunol. 1994; 152(4):1523-31. (Biology). View Reference
  8. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  9. Chattopadhyay PK, Yu J, Roederer M. A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profiles.. Nat Med. 2005; 11(10):1113-7. (Biology). View Reference
  10. Clinical and Laboratory Standards Institute. 2005. (Biology).
  11. Ding L, Green JM, Thompson CB, Shevach EM. B7/CD28-dependent and -independent induction of CD40 ligand expression.. J Immunol. 1995; 155(11):5124-32. (Biology). View Reference
  12. Durie FH, Foy TM, Masters SR, Laman JD, Noelle RJ. The role of CD40 in the regulation of humoral and cell-mediated immunity.. Immunol Today. 1994; 15(9):406-11. (Biology). View Reference
  13. Frentsch M, Arbach O, Kirchhoff D, et al. Direct access to CD4+ T cells specific for defined antigens according to CD154 expression.. Nat Med. 2005; 11(10):1118-24. (Biology). View Reference
  14. Fuleihan R, Ahern D, Geha RS. CD40 ligand expression is developmentally regulated in human thymocytes.. Clin Immunol Immunopathol. 1995; 76(1 Pt 1):52-8. (Biology). View Reference
  15. Gauchat JF, Aubry JP, Disanto J, et al. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1995:416-418.
  16. Han S, Hathcock K, Zheng B, Kepler TB, Hodes R, Kelsoe G. Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers.. J Immunol. 1995; 155(2):556-67. (Biology). View Reference
  17. Hollenbaugh D, Grosmaire LS, Kullas CD, et al. The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity.. EMBO J. 1992; 11(12):4313-21. (Biology). View Reference
  18. Lane P, Traunecker A, Hubele S, Inui S, Lanzavecchia A, Gray D. Activated human T cells express a ligand for the human B cell-associated antigen CD40 which participates in T cell-dependent activation of B lymphocytes.. Eur J Immunol. 1992; 22(10):2573-8. (Biology). View Reference
  19. Noelle RJ, Roy M, Shepherd DM, Stamenkovic I, Ledbetter JA, Aruffo A. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc Natl Acad Sci U S A. 1992; 89(14):6550-6554. (Biology). View Reference
  20. Noelle RJ. The role of gp39 (CD40L) in immunity.. Clin Immunol Immunopathol. 1995; 76(3 Pt 2):S203-7. (Biology). View Reference
  21. Ramesh N, Morio T, Fuleihan R, et al. CD40-CD40 ligand (CD40L) interactions and X-linked hyperIgM syndrome (HIGMX-1).. Clin Immunol Immunopathol. 1995; 76(3 Pt 2):S208-13. (Biology). View Reference
  22. Roy M, Waldschmidt T, Aruffo A, Ledbetter JA, Noelle RJ. The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells. J Immunol. 1993; 151(5):2497-2510. (Biology). View Reference
  23. Spriggs MK, Armitage RJ, Strockbine L, et al. Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion.. J Exp Med. 1992; 176(6):1543-50. (Biology). View Reference
View All (23) View Less
340477 Rev. 1

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