Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Germany (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      PE Mouse Anti-Human CD154
      Product Details
      Down Arrow Up Arrow


      BD™
      CD40LG; CD40 ligand; CD40L; gp39; TNFSF5; T-BAM; TRAP
      Human
      Mouse BALB/c IgG1, κ
      sgp39-CD8 and Activated Human PBL
      Flow cytometry
      12.5 μg/mL
      20 μL
      959
      Phosphate buffered saline with gelatin and 0.1% sodium azide.
      RUO (GMP)


      Preparation And Storage

      Store vials at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

      Show More blue down arrow Show Less blue up arrow
      340477 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      89-76

      CD154, clone 89-76, is derived from hybridization of mouse NS-1 cells with spleen cells from BALB/c mice immunized with sgp39-CD8 and activated human peripheral blood lymphocytes (PBLs).

      CD154 (CD40 ligand or CD40L) recognizes a 39-kilodalton (kDa) antigen that is a member of the tumor necrosis factor (TNF) receptor family. CD154 is transiently expressed on activated CD4 T lymphocytes. The expression of CD154 by activated T-helper cells triggers B-cell cycling through binding to CD40. This co-receptor interaction is required for B-lymphocyte maturation, response to T-dependent ligands, and isotype switching. T-lymphocyte proliferation and generation of cytotoxic T lymphocytes (CTLs) is associated with the presence of CD154. Defects in the CD154 gene are associated with the X-linked hyper IgM syndrome.

      340477 Rev. 1
      Format Details
      Down Arrow Up Arrow
      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      340477 Rev.1
      Citations & References
      Down Arrow Up Arrow

      Development References (23)

      1. Armitage RJ, Fanslow WC, Strockbine L, et al. Molecular and biological characterization of a murine ligand for CD40.. Nature. 1992; 357(6373):80-2. (Biology). View Reference
      2. Aruffo A, Farrington M, Hollenbaugh D, et al. The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome.. Cell. 1993; 72(2):291-300. (Biology). View Reference
      3. Ashokkumar C, Talukdar A, Sun Q, et al. Allospecific CD154+ T cells associate with rejection risk after pediatric liver transplantation.. Am J Transplant. 2009; 9(1):179-91. (Biology). View Reference
      4. Axcrona K, Gray D, Leanderson T. Regulation of B cell growth and differentiation via CD21 and CD40. Eur J Immunol. 1996; 26(9):2203-2207. (Biology). View Reference
      5. Bour-Jordan H, Bluestone JA. Regulating the regulators: costimulatory signals control the homeostasis and function of regulatory T cells.. Immunol Rev. 2009; 229(1):41-66. (Biology). View Reference
      6. Carbone A, Gloghini A, Gruss HJ, Pinto A. CD40 ligand is constitutively expressed in a subset of T cell lymphomas and on the microenvironmental reactive T cells of follicular lymphomas and Hodgkin's disease.. Am J Pathol. 1995; 147(4):912-22. (Biology). View Reference
      7. Cayabyab M, Phillips JH, Lanier LL. CD40 preferentially costimulates activation of CD4+ T lymphocytes.. J Immunol. 1994; 152(4):1523-31. (Biology). View Reference
      8. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
      9. Chattopadhyay PK, Yu J, Roederer M. A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profiles.. Nat Med. 2005; 11(10):1113-7. (Biology). View Reference
      10. Clinical and Laboratory Standards Institute. 2005. (Biology).
      11. Ding L, Green JM, Thompson CB, Shevach EM. B7/CD28-dependent and -independent induction of CD40 ligand expression.. J Immunol. 1995; 155(11):5124-32. (Biology). View Reference
      12. Durie FH, Foy TM, Masters SR, Laman JD, Noelle RJ. The role of CD40 in the regulation of humoral and cell-mediated immunity.. Immunol Today. 1994; 15(9):406-11. (Biology). View Reference
      13. Frentsch M, Arbach O, Kirchhoff D, et al. Direct access to CD4+ T cells specific for defined antigens according to CD154 expression.. Nat Med. 2005; 11(10):1118-24. (Biology). View Reference
      14. Fuleihan R, Ahern D, Geha RS. CD40 ligand expression is developmentally regulated in human thymocytes.. Clin Immunol Immunopathol. 1995; 76(1 Pt 1):52-8. (Biology). View Reference
      15. Gauchat JF, Aubry JP, Disanto J, et al. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1995:416-418.
      16. Han S, Hathcock K, Zheng B, Kepler TB, Hodes R, Kelsoe G. Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers.. J Immunol. 1995; 155(2):556-67. (Biology). View Reference
      17. Hollenbaugh D, Grosmaire LS, Kullas CD, et al. The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity.. EMBO J. 1992; 11(12):4313-21. (Biology). View Reference
      18. Lane P, Traunecker A, Hubele S, Inui S, Lanzavecchia A, Gray D. Activated human T cells express a ligand for the human B cell-associated antigen CD40 which participates in T cell-dependent activation of B lymphocytes.. Eur J Immunol. 1992; 22(10):2573-8. (Biology). View Reference
      19. Noelle RJ, Roy M, Shepherd DM, Stamenkovic I, Ledbetter JA, Aruffo A. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc Natl Acad Sci U S A. 1992; 89(14):6550-6554. (Biology). View Reference
      20. Noelle RJ. The role of gp39 (CD40L) in immunity.. Clin Immunol Immunopathol. 1995; 76(3 Pt 2):S203-7. (Biology). View Reference
      21. Ramesh N, Morio T, Fuleihan R, et al. CD40-CD40 ligand (CD40L) interactions and X-linked hyperIgM syndrome (HIGMX-1).. Clin Immunol Immunopathol. 1995; 76(3 Pt 2):S208-13. (Biology). View Reference
      22. Roy M, Waldschmidt T, Aruffo A, Ledbetter JA, Noelle RJ. The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells. J Immunol. 1993; 151(5):2497-2510. (Biology). View Reference
      23. Spriggs MK, Armitage RJ, Strockbine L, et al. Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion.. J Exp Med. 1992; 176(6):1543-50. (Biology). View Reference
      View All (23) View Less
      340477 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

      Website Feedback