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IgM-PerCP-Cyanine5.5
Product Details
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BD Cytognos™
IgM
Human
Mouse IgG1
Human IgM+ memory B cells and plasma cells
3 µl/test
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


cyt-igmc2 Rev. 1
Antibody Details
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MHM-88

IgM antibody, clone MHM-88, is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgM expressing cells. Monomeric IgM is always the first form and isotype of Ig generated by naive B cells. Following its initial activation by antigen, the naive B cell proliferates and differentiates, and its progeny produce the pentameric secreted form of IgM. Thus, it is IgM antibodies that are expressed first in any primary immune response.

cyt-igmc2 Rev. 1
Format Details
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PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
cyt-igmc2 Rev.1
Citations & References
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cyt-igmc2 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.