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Anti-HLA-DR FITC
Product Details
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BD™
MHC class II antigen; HLA class II histocompatibility antigen
Human
Mouse IgG2a, κ
Human lymphoblastoid B-cell line RPMI 8866
Flow cytometry
25 μg/mL
20 μL
Phosphate buffered saline with gelatin and 0.1% sodium azide.
CE_IVD


Preparation And Storage

The antibody reagent is stable until the expiration date shown on the label when stored at 2°C to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the outside of the reagent vial dry.

Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration.

347400 Rev. 1
Antibody Details
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L243

Anti–HLA-DR is intended for in vitro diagnostic use in the identification of cells expressing the HLA-DR antigen, using a BD FACS™ brand flow cytometer.

The flow cytometer must be equipped to detect light scatter and the appropriate fluorescence, and be equipped with appropriate software (such as BD CellQuest™, BD CellQuest™ Pro, BD FACSDiva™, or BD FACSCanto™ clinical software) for data acquisition and analysis. See your instrument user’s guide for instructions.

347400 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
347400 Rev.1
Citations & References
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View product citations for antibody "347400" on CiteAb

Development References (31)

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  3. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
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  7. Dorak MT, Lawson T, Machulla HK, Darke C, Mills KI, Burnett AK. Unravelling an HLA-DR association in childhood acute lymphoblastic leukemia. Blood. 1999; 94:694-700. (Biology).
  8. Edwards JA, Durant BM, Jones DB, Evans PR, Smith JL. Differential expression of HLA class II antigens in fetal human spleen: relationship of HLA-DP, DQ, and DR to immunoglobulin expression.. J Immunol. 1986; 137(2):490-7. (Biology). View Reference
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  10. Geisberg M, Stole E, Knowles R. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press Inc; 1989:729-736.
  11. Hulstaert F, Hannet I, Deneys V, et al. Age-related changes in human blood lymphocyte subpopulations, II: Varying kinetics of percentage and absolute count measurements. Clin Immunol Immunopathol. 1994; 70:152-158. (Biology).
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  15. Levacher M, Tallet S, Dazza MC, Dournon E, Rouveix B, Pocidalo JJ. T activation marker evaluation in ARC patients treated with AZT. Comparison with CD4+ lymphocyte count in non-progressors and progressors towards AIDS.. Clin Exp Immunol. 1990; 81(2):177-82. (Biology). View Reference
  16. Macedo A, Orfao A, Gonzalez M, et al. Immunological detection of blast cell subpopulations in acute myeloblastic leukemia at diagnosis: implications for minimal residual disease studies. Leukemia. 1995; 9:993-998. (Biology).
  17. O'Gormon MRG, Millard DD, Lowder JN, Yogev R. Lymphocyte subpopulations in healthy 1–3-day-old infants. Cytometry. 1998; 34:235-241. (Biology).
  18. Paietta E, Andersen J, Gallagher R, et al. The immunophenotype of acute promyelocytic leukemia (APL): an ECOG study. Leukemia. 1994; 8:1108-1112. (Biology).
  19. Porwit-MacDonald A, Janossy G, Ivory K, et al. Leukemia-associated changes identified by quantitative flow cytometry, IV: CD34 overexpression in acute myelogenous leukemia M2 with t(8;21). Blood. 1996; 87:1162-1169. (Biology).
  20. Schuerch CI, Fleetwood M, Glidewell O, Goodspeed N, Maier B. Lymphocyte subsets and activation antigens in a reference population: a flow cytometric study using single and double antibody staining. Immunol Invest. 1987; 16:345-360. (Biology).
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View All (31) View Less
347400 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For In Vitro Diagnostic Use.

 

23-22942-00

Documents are subject to revision without notice. Please verify you have the correct revision of the document, and always refer back to BD's eIFU website for the latest and most up to date information.