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Purified Mouse Anti-Rat CD4
Purified Mouse Anti-Rat CD4

Two-color analysis of the expression of CD4 on rat splenic leukocytes. Lewis splenocytes were simultaneously stained with purified anti-rat CD4 mAb clone OX-35 (right panel) and PE-conjugated anti-rat CD3 mAb clone G4.18 (Cat. No. 554833), followed by FITC-conjugated anti-mouse IgG2a mAb R19-15 (Cat. No. 553390). The CD3-negative CD4-dim cells are the monocyte/macrophage population. Flow cytometry was performedon a BD FACScan™ flow cytometry system.

Two-color analysis of the expression of CD4 on rat splenic leukocytes. Lewis splenocytes were simultaneously stained with purified anti-rat CD4 mAb clone OX-35 (right panel) and PE-conjugated anti-rat CD3 mAb clone G4.18 (Cat. No. 554833), followed by FITC-conjugated anti-mouse IgG2a mAb R19-15 (Cat. No. 553390). The CD3-negative CD4-dim cells are the monocyte/macrophage population. Flow cytometry was performedon a BD FACScan™ flow cytometry system.

Product Details
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BD Pharmingen™
Rat (QC Testing)
Mouse BALB/c IgG2a, κ
Rat T-cell blasts
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Depletion, Immunoprecipitation (Reported)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Recommended Assay Procedure:

Caution: Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals.  Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer.  Since endotoxin may also effect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554835 Rev. 15
Antibody Details
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OX-35

The OX-35 clone recognizes the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, some dendritic cells, and microglia. CD4 is an antigen coreceptor on the T-cell surface that interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through it's association with the T-cell receptor complex and protein tyrosine kinase Lck. The OX-35 clone has been reported to bind to a different epitope of CD4 than that recognized by the W3/25 and OX-38 clones.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

554835 Rev. 15
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
554835 Rev.15
Citations & References
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Development References (9)

  1. Bañuls MP, Alvarez A, Ferrero I, Zapata A, Ardavin C. Cell-surface marker analysis of rat thymic dendritic cells. Immunology. 1993; 79(2):298-304. (Biology). View Reference
  2. Bierer BE, Sleckman BP, Ratnofsky SE, Burakoff SJ. The biologic roles of CD2, CD4, and CD8 in T-cell activation. Annu Rev Immunol. 1989; 7:579-599. (Biology). View Reference
  3. Ford AL, Foulcher E, Goodsall AL, Sedgwick JD. Tissue digestion with dispase substantially reduces lymphocyte and macrophage cell-surface antigen expression. J Immunol Methods. 1996; 194(1):71-75. (Biology: Depletion). View Reference
  4. Janeway CA Jr. The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation. Annu Rev Immunol. 1992; 10:645-674. (Biology). View Reference
  5. Jefferies WA, Green JR, Williams AF. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages.. J Exp Med. 1985; 162:117-127. (Immunogen: Immunoprecipitation).
  6. Liu L, Zhang M, Jenkins C, MacPherson GG. Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression. J Immunol. 1998; 161(3):1146-1155. (Biology). View Reference
  7. McElwee KJ, Spiers EM, Oliver RF. Partial restoration of hair growth in the DEBR model for Alopecia areata after in vivo depletion of CD4+ T cells. Br J Dermatol. 1999; 140(3):432-437. (Clone-specific: Depletion). View Reference
  8. Morrison WJ, Kennedy NJ, Offner H, Vandenbark AA. Effects of anti-CD4 antibody: enhancement of lymph node PPD-memory T cell response. Cell Immunol. 1995; 163(1):106-112. (Clone-specific: Depletion). View Reference
  9. Wang CC, Wu CH, Shieh JY, Wen CY, Ling EA. Immunohistochemical study of amoeboid microglial cells in fetal rat brain. J Anat. 1996; 189(3):567-574. (Biology). View Reference
View All (9) View Less
554835 Rev. 15

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.