Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Switzerland (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
      Alert Icon
      RB545 Mouse Anti-Human CD24
      RB545 Mouse Anti-Human CD24

      Multiparameter flow cytometric analysis of CD24 expression on Human peripheral blood leukocytes.  Fresh whole blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/564220) and then stained with APC Mouse Anti-Human CD19 antibody (Cat. No. 555415/561742; Top Plots) and with either BD Horizon™ RB545 Mouse IgG2a, κ Isotype Control (Cat. No. 569277; Left Plots) or BD Horizon™ RB545 Mouse Anti-Human CD24 antibody (Cat. No. 569746/569774; Right Plots). The bivariate pseudocolor density plots showing the correlated expression of CD24 (or Ig Isotype control staining) versus CD19 (Top Plots) or side light-scatter (SSC-A) signals (Bottom Plots) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Top Plots) or leukocyte populations (Bottom Plots). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer and spectrally unmixed using FlowJo™ v10.8 software.

      RB545 Mouse Anti-Human CD24

      Multiparameter flow cytometric analysis of CD24 expression on Human peripheral blood leukocytes.  Fresh whole blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/564220) and then stained with APC Mouse Anti-Human CD19 antibody (Cat. No. 555415/561742; Top Plots) and with either BD Horizon™ RB545 Mouse IgG2a, κ Isotype Control (Cat. No. 569277; Left Plots) or BD Horizon™ RB545 Mouse Anti-Human CD24 antibody (Cat. No. 569746/569774; Right Plots). The bivariate pseudocolor density plots showing the correlated expression of CD24 (or Ig Isotype control staining) versus CD19 (Top Plots) or side light-scatter (SSC-A) signals (Bottom Plots) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Top Plots) or leukocyte populations (Bottom Plots). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer and spectrally unmixed using FlowJo™ v10.8 software.

      Product Details
      Down Arrow Up Arrow


      BD Horizon™
      Heat Stable Antigen Homologue (HSA); Ba-1; CD24A
      Human (QC Testing)
      Mouse IgG2a, κ
      Flow cytometry (Routinely Tested)
      5 µl/test
      IV B243; V CD24.5
      100133941
      AB_3685285
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      9. For U.S. patents that may apply, see bd.com/patents.
      Show More blue down arrow Show Less blue up arrow
      569746 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      ML5

      The ML5 monoclonal antibody specifically binds to CD24 which is also known as CD24A, signal transducer CD24, small cell lung carcinoma cluster 4 antigen, or BA-1. CD24 is a 35-70 kDa glycophosphatidylinositol (GPI)-linked glycoprotein whose glycosylation pattern is highly variable and cell-type dependent. CD24 is expressed on neutrophils, eosinophils, dendritic cells, neural cells, epithelial cells, muscle cells, and many types of cancer cells. CD24 functions as an adhesion receptor. Several different ligands have been identified for CD24 including CD62P (P-selectin) which is expressed on activated platelets and activated endothelium. CD24 is variably expressed on all B lineage cells, except plasma cells, and can play a role in regulating the activation, proliferation or differentiation of these cells.

      569746 Rev. 1
      Format Details
      Down Arrow Up Arrow
      RB545
      The BD Horizon RealBlue™ 545 (RB545) fluorochrome is part of the BD family of blue (488-nm) laser dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 496-nm and an emission maximum (Em Max) at 545-nm. Driven by BD innovation, RB545 can be used on spectral cytometers and is designed to be excited by the blue (488-nm) laser with minimal excitation by the 561-nm yellow-green laser. RB545 has minimal spillover into yellow-green detectors and a brightness level similar to FITC.  Given its unique emission max, on a spectral instrument RB545 can be used simultaneously with FITC and PE to provide an additional color off of the blue laser. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
      altImg
      RB545
      Blue 488 nm
      496 nm
      545 nm
      569746 Rev.1
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "569746" on CiteAb

      Development References (6)

      1. Chtanova T, Tangye SG, Newton R, et al. T follicular helper cells express a distinctive transcriptional profile, reflecting their role as non-Th1/Th2 effector cells that provide help for B cells. J Immunol. 2004; 173(1):68-78. (Clone-specific: Flow cytometry). View Reference
      2. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. B-cell antigens: CD24. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:82-83.
      3. McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:1-1050.
      4. Salamone MC, Rosselot C, Salamone GV, Barboza M, Kado M, Fainboim L. Antibodies recognizing CD24 LAP epitope on human T cells enhance CD28 and IL-2 T cell proliferation . J Leukoc Biol. 2001; 69(2):215-223. (Clone-specific: Flow cytometry). View Reference
      5. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
      6. Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
      View All (6) View Less
      569746 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.