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R718 Rat Anti-Mouse CD98
R718 Rat Anti-Mouse CD98
Flow cytometric analysis of CD98 expression on unstimulated or stimulated Mouse splenic leucocytes. BALB/c mouse splenic leucocytes were tested fresh (Unstimulated; Left Panel) or 48 hours after Concanavalin A (Con A) mitogenic stimulation (Stimulated; Right Panel). The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142) and stained with either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; dotted line histograms) or BD Horizon™ R718  Rat Anti-Mouse CD98 antibody (Cat No. 568199/568200; solid line histograms) at 0.5 µg/test. The fluorescence histograms showing CD98 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mouse leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of CD98 expression on unstimulated or stimulated Mouse splenic leucocytes. BALB/c mouse splenic leucocytes were tested fresh (Unstimulated; Left Panel) or 48 hours after Concanavalin A (Con A) mitogenic stimulation (Stimulated; Right Panel). The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142) and stained with either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; dotted line histograms) or BD Horizon™ R718  Rat Anti-Mouse CD98 antibody (Cat No. 568199/568200; solid line histograms) at 0.5 µg/test. The fluorescence histograms showing CD98 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mouse leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Horizon™
Slc3a2; Cd98; 4F2; 4F2HC; CD98 heavy chain; Ly-10; Ly-m10; Ly10
Mouse (QC Testing)
Rat OFA, also known as Outbred OFA IgG2a, κ
EL4-6.1 Thymoma Cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
  7. Alexa Fluor™ is a trademark of Life Technologies Corporation.
568200 Rev. 1
Antibody Details
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RL388

The RL388 monoclonal antibody recognizes CD98 which is also known as the 4F2 antigen and Ly-10. CD98 is a disulfide-linked heterodimer comprised of an ~86 kDa single-pass type II heavy chain glycoprotein that associates with a ~39-kDa multi-pass, nonglycosylated light chain which belongs to the L-type amino transporter family. The RL388 antibody specifically binds to an epitope present on the CD98 heavy chain (CD98hc, also known as the 4F2 cell-surface antigen heavy chain or 4F2hc) which is encoded by Slc3a2 [solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2]. CD98 is variably expressed on different cell types including T cells, B cells, and NK cells as well as monocytes, granulocytes, platelets, and fibroblasts. CD98 serves as an activation marker for T and B cells since its expression is upregulated upon cellular activation. CD98 regulates cellular growth, proliferation, and survival through CD98hc interactions with β integrins and by CD98 light chain-mediated amino acid transport.

568200 Rev. 1
Format Details
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R718
The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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R718
Red 627-640 nm
695 nm
718 nm
568200 Rev.1
Citations & References
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View product citations for antibody "568200" on CiteAb

Development References (7)

  1. Cantor J, Browne CD, Ruppert R, et al. CD98hc facilitates B cell proliferation and adaptive humoral immunity. Nat Immunol. 2009; 10(4):412-419. (Clone-specific: Flow cytometry). View Reference
  2. Cantor JM, Ginsberg MH. CD98 at the crossroads of adaptive immunity and cancer. J Cell Sci. 2012; 125(Pt 6):1373-1382. (Biology). View Reference
  3. Ernst DN, McQuitty DN, Weigle WO, Hobbs MV. Expression of membrane activation antigens on murine B lymphocytes stimulated with lipopolysaccharide. Cell Immunol. 1988; 114(1):161-173. (Clone-specific: Flow cytometry). View Reference
  4. Ernst DN, Weigle WO, McQuitty DN, Rothermel AL, Hobbs MV. Stimulation of murine T cell subsets with anti-CD3 antibody. Age-related defects in the expression of early activation molecules. J Immunol. 1989; 142(5):1413-1421. (Clone-specific: Flow cytometry). View Reference
  5. Luscher B, Rousseaux M, Lees R, MacDonald HR, Bron C. Cell surface glycoproteins involved in the stimulation of interleukin 1-dependent interleukin 2 production by a subline of EL4 thymoma cells. II. Structure, biosynthesis, and maturation. J Immunol. 1985; 135(6):3951-3957. (Clone-specific: Immunoprecipitation, Radioimmunoassay). View Reference
  6. MacDonald HR, Lees RK, Bron C. Cell surface glycoproteins involved in the stimulation of interleukin 1-dependent interleukin 2 production by a subline of EL4 thymoma cells. I. Functional characterization by monoclonal antibodies. J Immunol. 1985; 135(6):3944-3950. (Immunogen: (Co)-stimulation, Flow cytometry, Functional assay, Radioimmunoassay). View Reference
  7. Sato Y, Heimeier RA, Li C, Deng C, Shi YB. Extracellular domain of CD98hc is required for early murine development.. Cell Biosci. 2011; 1(1):7. (Biology). View Reference
View All (7) View Less
568200 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.