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Purified Rabbit Anti-Human CD88
Product Details
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BD Pharmingen™
C5a Receptor
Human (QC Testing)
Rabbit IgG
Human C5aR sequence aa. 9-29
Flow cytometry (Routinely Tested)
20 µl
AB_397199
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Immunofluorescent Staining and Flow Cytometric Analysis: The C85-4124 antibody is useful for flow cytometric analysis of human C5aR expression. The expression level of human C5aR detected by C85-4124 varies among donors tested. The purified antibody can be used in conjunction with biotinylated goat anti-rabbit Ig and streptavidin-phycoerythrin (Cat. No. 554061) in a three-step staining procedure to amplify immunofluorescent signals.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
559159 Rev. 1
Antibody Details
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C85-4124

The rabbit monoclonal antibody C85-4124 reacts with human CD88, which is also known as C5a anaphylatoxin Receptor (C5a receptor, C5aR). CD88 (C5aR) is a seven-transmembrane-spanning G-protein coupled receptor. Its ligand, C5a/C5a-desArg, is the 10 kDa cleavage product of serum protein C5, generated upon Complement System activation. Ligand binding activates the receptor, and mediates lysosomal enzyme release, cytokine and superoxide production, and chemotaxis. Ligand-occupied receptors get internalized (downregulated). Main cellular expression of CD88 (C5aR) includes granulocytes, monocytes, dendritic cells, astrocytes, and microglia. The extracellular, N-terminal 9-29 amino acids of the human C5aR sequence was used as an immunogen to generate C85-4124 monoclonal antibody from rabbits.

559159 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
559159 Rev.1
Citations & References
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Development References (9)

  1. Fayyazi A, Sandau R, Duong LQ. C5a receptor and interleukin-6 are expressed in tissue macrophages and stimulated keratinocytes but not in pulmonary and intestinal epithelial cells. Am J Pathol. 1999; 154(2):495-501. (Biology). View Reference
  2. Floreani AA, Heires AJ, Welniak LA. Expression of receptors for C5a anaphylatoxin (CD88) on human bronchial epithelial cells: enhancement of C5a-mediated release of IL-8 upon exposure to cigarette smoke. J Immunol. 1998; 160(10):5073-5081. (Biology). View Reference
  3. Fukuoka Y, Ember JA, Yasui A, Hugli TE. Cloning and characterization of the guinea pig C5a anaphylatoxin receptor: interspecies diversity among the C5a receptors. Int Immunol. 1998; 10(3):275-283. (Biology). View Reference
  4. Füreder W, Agis H, Willheim M. Differential expression of complement receptors on human basophils and mast cells. Evidence for mast cell heterogeneity and CD88/C5aR expression on skin mast cells. J Immunol. 1995; 155(6):3152-3160. (Biology). View Reference
  5. Gasque P, Singhrao SK, Neal JW, Götze O, Morgan BP. Expression of the receptor for complement C5a (CD88) is up-regulated on reactive astrocytes, microglia, and endothelial cells in the inflamed human central nervous system. Am J Pathol. 1997; 150(1):31-41. (Biology). View Reference
  6. Haviland DL, McCoy RL, Whitehead WT. Cellular expression of the C5a anaphylatoxin receptor (C5aR): demonstration of C5aR on nonmyeloid cells of the liver and lung. J Immunol. 1995; 154(4):1861-1869. (Biology). View Reference
  7. Morgan EL, Ember JA, Sanderson SD, et al. Anti-C5a receptor antibodies. Characterization of neutralizing antibodies specific for a peptide, C5aR-(9-29), derived from the predicted amino-terminal sequence of the human C5a receptor. J Immunol. 1993; 151(1):377-388. (Biology). View Reference
  8. Spieker-Polet H, Sethupathi P, Yam PC, Knight KL. Rabbit monoclonal antibodies: generating a fusion partner to produce rabbit-rabbit hybridomas. Proc Natl Acad Sci U S A. 1995; 92(20):9348-9352. (Biology). View Reference
  9. Zahn S, Zwirner J, Spengler HP, Götze O. Chemoattractant receptors for interleukin-8 and C5a: expression on peripheral blood leukocytes and differential regulation on HL-60 and AML-193 cells by vitamin D3 and all-trans retinoic acid. J Immunol. 1997; 27(4):935-940. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.