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Purified NA/LE Rat Anti-Human CD210a
Purified NA/LE Rat Anti-Human CD210a

Flow cytometric profile of CD210a expression on human peripheral blood lymphocytes. Human whole blood was stained with either Purified NA/LE Rat IgG2a, κ Isotype Control (Cat. No. 555840; dashed line histogram) or Purified NA/LE Rat Anti-Human CD210a (Cat. No. 556011; solid line histogram). Three-step staining was carried out with Biotin Goat Anti-Rat Ig (Cat. No. 554014) and PE Streptavidin (Cat. No. 554061). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACSCanto™ System.

Flow cytometric profile of CD210a expression on human peripheral blood lymphocytes. Human whole blood was stained with either Purified NA/LE Rat IgG2a, κ Isotype Control (Cat. No. 555840; dashed line histogram) or Purified NA/LE Rat Anti-Human CD210a (Cat. No. 556011; solid line histogram). Three-step staining was carried out with Biotin Goat Anti-Rat Ig (Cat. No. 554014) and PE Streptavidin (Cat. No. 554061). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACSCanto™ System.

Product Details
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BD Pharmingen™
CD210; CDW210A; IL10RA; IL-10RA; IL-10R-A; IL10R; HIL-10R; IL-10R1; I10R1
Human (QC Testing)
Rat F344, also known as Fischer, CDF IgG2a, κ
Human IL-10R alpha Recombinant Protein
Flow cytometry (Routinely Tested)
1.0 mg/ml
IX 30
3587
AB_396289
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
556011 Rev. 3
Antibody Details
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3F9

The 3F9 monoclonal antibody specifically binds to CD210a, which is also known as, Interleukin-10 Receptor subunit alpha (IL-10R subunit alpha/IL-10Rα/IL10RA), or Interleukin-10 receptor subunit 1 (IL-10R1). CD210a is a 90-110 kDa type I transmembrane glycoprotein that belongs to the type II cytokine receptor family. CD210a combines with IL-10 Receptor subunit beta (IL-10Rβ/IL10RB/CDw210b) to form the IL-10 Receptor complex (IL-10Rα/IL-10Rβ) that can bind IL-10 and transduce signals intracellularly through the JAK/STAT pathway. IL-10 can suppress antigen presentation and the expression of proinflammatory type-1 immune responses while promoting type-2 immune responses. CD210a is expressed on T cells, B cells, NK cells, monocyte, macrophages and dendritic cells. Clone 3F9 is specific for human CD210a and its binding to the receptor can be blocked by recombinant human IL-10 (rhIL-10) protein.

556011 Rev. 3
Format Details
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NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
NA/LE
556011 Rev.3
Citations & References
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Development References (4)

  1. Callard R, Gearing A. Callard R, Gearing A. The Cytokine Facts Book. San Diego: Academic Press; 1994.
  2. Carson WE, Lindemann MJ, Baiocchi R, et al. The functional characterization of interleukin-10 receptor expression on human natural killer cells. Blood. 1995; 85(12):3577-3585. (Biology). View Reference
  3. Liu Y, Wei SH, Ho AS, de Waal Malefyt R, Moore KW. Expression cloning and characterization of a human IL-10 receptor. J Immunol. 1995; 152(4):1821-1829. (Biology). View Reference
  4. Liu Y, de Waal Malefyt R, Briere F, et al. The EBV IL-10 homologue is a selective agonist with impaired binding to the IL-10 receptor.. J Immunol. 1997; 158(2):604-13. (Biology). View Reference
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556011 Rev. 3

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.