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Purified NA/LE Mouse Anti-Human CD206
Purified NA/LE Mouse Anti-Human CD206

Flow cytometric analysis for CD206.  Human peripheral blood mononuclear cells (PBMC), stimulated with GM-CSF for 3 days, were stained either with the Purified NA/LE Mouse Anti-Human CD206 antibody (solid line) or a Mouse IgG1 isotype control (dotted line) followed by a FITC Goat Anti-Mouse IgG/IgM polyclonal secondary antibody.  Activated monocytes (large cell population) were gated and analyzed on a FACScan (BDIS, San Jose, CA).

Flow cytometric analysis for CD206.  Human peripheral blood mononuclear cells (PBMC), stimulated with GM-CSF for 3 days, were stained either with the Purified NA/LE Mouse Anti-Human CD206 antibody (solid line) or a Mouse IgG1 isotype control (dotted line) followed by a FITC Goat Anti-Mouse IgG/IgM polyclonal secondary antibody.  Activated monocytes (large cell population) were gated and analyzed on a FACScan (BDIS, San Jose, CA).

Product Details
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BD Pharmingen™
Macrophage mannose receptor; MMR; MRC1; CLEC13D
Human (QC Testing)
Mouse IgG1, κ
Human Placental Macrophage Mannose Receptor
Flow cytometry (Routinely Tested)
1.0 mg/ml
VII 70318
4360
AB_396248
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555952 Rev. 9
Antibody Details
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19.2

The 19.2 monoclonal antibody specifically binds to CD206 which is also known as the macrophage mannose receptor (MMR) or C-type lectin domain family 13 member D (CLEC13D). CD206 is a type I transmembrane glycoprotein of approximately 162 kDa which binds to glycoconjugates containing mannose, fucose, or N-acetylglucosamine. These carbohydrates are present on the surface of many microorganisms and enable the macrophage to bind to microorganisms through the MMR and internalize them during the process of phagocytosis. CD206 is expressed on human macrophages, endothelial cells, and cultured dendritic cells. It is not detected on resting monocytes. Mannose receptor expression is upregulated on peripheral blood mononuclear cells following 3 day incubation with GM-CSF.

555952 Rev. 9
Format Details
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NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
NA/LE
555952 Rev.9
Citations & References
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Development References (2)

  1. Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002.
  2. Pontow SE, Kery V, Stahl PD. Mannose receptor. Int Rev Cytol. 1992; 137(B):221-244. (Biology). View Reference
555952 Rev. 9

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.