Skip to main content Skip to navigation
Purified Mouse Anti-Rat β2 Microglobulin
Product Details
Down Arrow Up Arrow


BD Pharmingen™
Rat (QC Testing)
Mouse BALB/c IgG1, κ
Cultured Lewis rat microglial cells
Flow cytometry (Routinely Tested), Immunohistochemistry, Western blot (Reported)
0.5 mg/ml
AB_397112
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
558765 Rev. 4
Antibody Details
Down Arrow Up Arrow
TLD-3H12B

The TLD-3H12B monoclonal antibody specifically recognizes β2 microglobulin, a 12 kDa protein consisting of a single immunoglobulin constant-region domain. β2 microglobulin is noncovalently associated with the MHC class I heavy chain and CD1. It is expressedon most nucleated cells and erythrocytes.

558765 Rev. 4
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
558765 Rev.4
Citations & References
Down Arrow Up Arrow

Development References (2)

  1. Ichimiya S, Kikuchi K, Matsuura A. Structural analysis of the rat homologue of CD1. Evidence for evolutionary conservation of the CD1D class and widespread transcription by rat cells. J Immunol. 1994; 153(3):1112-1123. (Biology). View Reference
  2. Kroese FGM. Immunology of the Rat. In: Pastoret PP, Griebel P, Bazin H, Govaerts A, ed. Handbook of Vertebrae Immunology. Academic Press; 1998:137-222.
558765 Rev. 4

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.