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BD Pharmingen™ Purified Mouse Anti-Human HLA-DM
Clone MaP.DM1 (RUO)
Profile of intracellular staining of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA).
Profile of intracellular staining of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA).
Profile of intracellular staining of peripheral blood monocytes analyzed on a FACScan (BDIS, San Jose, CA).
Profile of intracellular staining of peripheral blood monocytes analyzed on a FACScan (BDIS, San Jose, CA).
Profile of intracellular staining of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA).
Profile of intracellular staining of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA).
Profile of intracellular staining of peripheral blood monocytes analyzed on a FACScan (BDIS, San Jose, CA).
Profile of intracellular staining of peripheral blood monocytes analyzed on a FACScan (BDIS, San Jose, CA).
Product Details
BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Intracellular staining (flow cytometry) (Routinely Tested)
0.5 mg/ml
AB_396271
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
Fixation/Permeabilization Kit RUO
Size
250 Tests
Cat No.
554714
Purified Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
555746
FITC Goat Anti-Mouse IgG/IgM RUO
Size
0.5 mg
Cat No.
555988
555982 Rev. 5
Antibody Details
MaP.DM1
Reacts with human leukocyte antigen-DM (HLA-DM), a non-classical MHC class II molecule expressed in the cytoplasm of antigen presenting cells (APC). HLA-DM is composed of alpha and beta subunits which form a similar structure as the classical class II molecules. HLA-DM catalyzes the dissociation of CLIP from MHC class II-CLIP complexes in vitro and facilitates the binding of antigenic peptides. It also prevents self-antigens from becoming stably complexed with class II molecules and being presented to T cells.
555982 Rev. 5
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555982 Rev.5
Citations & References
View product citations for antibody "555982" on CiteAb
Development References (6)
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Denzin LK, Cresswell P. HLA-DM induces CLIP dissociation from MHC class II alpha beta dimers and facilitates peptide loading. Cell. 1995; 82(1):155-165. (Biology). View Reference
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Denzin LK, Hammond C, Cresswell P. HLA-DM interactions with intermediates in HLA-DR maturation and a role for HLA-DM in stabilizing empty HLA-DR molecules. J Exp Med. 1996; 184(186):2153-2165. (Biology). View Reference
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Denzin LK, Robbins NF, Carboy-Newcomb C, Cresswell P. Assembly and intracellular transport of HLA-DM and correction of the class II antigen-processing defect in T2 cells. Immunity. 1994; 1(7):595-606. (Biology). View Reference
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Kropshofer H, Hämmerling GJ, Vogt AB. How HLA-DM edits the MHC class II peptide repertoire: survival of the fittest. Immunol Today. 1997; 18(2):77-82. (Biology). View Reference
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Riberdy JM, Avva RR, Geuze HJ, Cresswell P. Transport and intracellular distribution of MHC class II molecules and associated invariant chain in normal and antigen-processing mutant cell lines. J Cell Biol. 1994; 125(126):1225-1237. (Biology). View Reference
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Riberdy JM, Newcomb JR, Surman MJ, Barbosa JA, Cresswell P. HLA-DR molecules from an antigen-processing mutant cell line are associated with invariant chain peptides. Nature. 1992; 360(6403):474-477. (Biology). View Reference
555982 Rev. 5
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.