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Purified Mouse Anti-Human fMLP receptor
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse C57BL/6 IgG1, κ
Human fMLP receptor Transfected Cell Line
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_396293
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
556015 Rev. 6
Antibody Details
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5F1

The 5F1 monoclonal antibody specifically binds to the N-formyl-leucyl-phenylalanine receptor  (fMLP receptor), which is also known as, Formyl peptide receptor 1 (FPR1). This 55-70 kDa receptor is expressed on neutrophils, monocytes, macrophages, and liver parenchymal cells. It is composed of seven hydrophobic membrane spanning regions and is a member of the rhodopsin superfamily of G protein-coupled receptors. N-formyl peptides interact with fMLP receptors and induce neutrophil chemotaxis, phagocytosis, production of superoxide radicals and release of proteolytic enzymes. The binding of the 5F1 antibody to the fMLP receptor can be blocked by fMLP peptide.

556015 Rev. 6
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556015 Rev.6
Citations & References
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Development References (3)

  1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  2. Lala A, Sojar HT, De Nardin E. Expression and purification of recombinant human N-formyl-L-leucyl-L-phenylalanine (FMLP) receptor: generation of polyclonal antibody against FMLP receptor. Biochem Pharmacol. 1997; 54(3):381-390. (Biology). View Reference
  3. Sengeløv H, Boulay F, Kjeldsen L, Borregaard N. Subcellular localization and translocation of the receptor for N-formylmethionyl-leucyl-phenylalanine in human neutrophils. Biochem J. 1994; 299(2):473-479. (Biology). View Reference
556015 Rev. 6

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.