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Purified Mouse Anti-Human E2A-Pbx1
Purified Mouse Anti-Human E2A-Pbx1

Profile of permeabilized 697 (pre-B cell leukemia cell line) fixed with BD FACS Lysing solution (Cat. No. 349202) and permeabilized by FACS Permeabilizing Solution (Cat. No. 340457) analyzed on a FACScan (BDIS, San Jose, CA).

Profile of permeabilized 697 (pre-B cell leukemia cell line) fixed with BD FACS Lysing solution (Cat. No. 349202) and permeabilized by FACS Permeabilizing Solution (Cat. No. 340457) analyzed on a FACScan (BDIS, San Jose, CA).

Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Intracellular staining (flow cytometry) (Routinely Tested)
0.5 mg/ml
AB_396299
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
556021 Rev. 5
Antibody Details
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G289-1

Reacts with E2A-Pbx1, a chimeric gene product of approximately 90 kDa which is the result of t(1,19) (q23;p13.3) chromosomal translocation. Sequences of Pbx1 from chromosome 1 are juxtaposed adjacent to sequences of the E2A gene on chromosome 19. The translocation results in the transcription of a unique chimeric protein E2A-Pbx1 with an amino acid sequences not present on E2A or Pbx alone. E2A-Pbx1 is found only in cells with the t(1,19) translocation. It is detectable in the nucleus of 697 cell line (a pre-B cell line). E2A-Pbx1 is considered to be an oncoprotein, however, the oncogenic mechanism has not been fully elucidated.

556021 Rev. 5
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556021 Rev.5
Citations & References
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Development References (4)

  1. Berendes P, Hoogeveen A, van Dijk M, van Denderen J, van Ewijk W. Specific immunologic recognition of the tumor-specific E2A-PBX1 fusion-point antigen in t(1;19)-positive pre-B cells.. Leukemia. 1995; 9(8):1321-1327. (Biology). View Reference
  2. Kamps MP, Look AT, Baltimore D. The human t(1;19) translocation in pre-B ALL produces multiple nuclear E2A-Pbx1 fusion proteins with differing transforming potentials. Genes Dev. 1991; 5(3):358-368. (Biology). View Reference
  3. Kamps MP, Murre C, Sun XH, Baltimore D. A new homeobox gene contributes the DNA binding domain of the t(1;19) translocation protein in pre-B ALL. Cell. 1990; 60(4):547-557. (Biology). View Reference
  4. Sang BC, Shi L, Dias P. Monoclonal antibodies specific to the acute lymphoblastic leukemia t(1;19)-associated E2A/pbx1 chimeric protein: characterization and diagnostic utility. Blood. 1997; 89(8):2909-2914. (Biology). View Reference
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556021 Rev. 5

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.