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PE Rat Anti-Mouse Ly-6D
PE Rat Anti-Mouse Ly-6D

Flow cytometric analysis of Ly-6D expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were simultaneously stained with PE Rat Anti-Mouse Ly-6D (Cat. No. 561149; left panel) or PE Rat IgG2c Isotype Control (Cat. No. 559841; right panel) and PerCP-Cy™5.5 Anti-Mouse CD3 (Cat. No. 561108). A two-color flow cytometric dot plot showing the correlated expression patterns of Ly-6D versus CD3 was derived from gated events with the forward and side light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD LSR™ II.

Flow cytometric analysis of Ly-6D expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were simultaneously stained with PE Rat Anti-Mouse Ly-6D (Cat. No. 561149; left panel) or PE Rat IgG2c Isotype Control (Cat. No. 559841; right panel) and PerCP-Cy™5.5 Anti-Mouse CD3 (Cat. No. 561108). A two-color flow cytometric dot plot showing the correlated expression patterns of Ly-6D versus CD3 was derived from gated events with the forward and side light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD LSR™ II.

Product Details
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BD Pharmingen™
Lymphocyte antigen 6 complex, locus D; Thb; Thymocyte B-cell antigen; Ly-61
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2c, κ
BALB/c mouse plasmacytoma MOPC-104E
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_10563763
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
561149 Rev. 2
Antibody Details
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49-H4

The 49-H4 antibody specifically binds to the ThB antigen (Ly-6D), a 15-kDa GPI-anchored protein of the Ly-6 Multigene Family. ThB has been detected on cortical thymocytes, small sIg- Thy-1- and sIg+ lymphoid cells in the bone marrow, thymic medullary epithelial cells, all epidermal layers (except the stratum corneum), and on peripheral B lymphocytes (but not peripheral T lymphocytes).  There is strain-to-strain variation in ThB antigen expression on splenic B cells. Strains with the Ly-6.2 haplotype (e.g., AKR, C57BL, DBA/2, SJL, SWR) stain more intensely with anti-ThB reagents than Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, NZB); B cells of Ly-6.1 and Ly-6.2 hybrids stain with intermediate intensity.  The proportion of ThB+ B cells to thymocytes do not differ significantly among strains.

561149 Rev. 2
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
561149 Rev.2
Citations & References
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Development References (6)

  1. Boyd RL, Tucek CL, Godfrey DI. The thymic microenvironment. Immunol Today. 1993; 14(9):445-459. (Biology). View Reference
  2. Eckhardt LA, Herzenberg LA. Monoclonal antibodies to ThB detect close linkage of Ly-6 and a gene regulating ThB expression. Immunogenetics. 1980; 11(3):275-291. (Clone-specific: Cytotoxicity). View Reference
  3. Godfrey DI, Izon DJ, Tucek CL, Wilson TJ, Boyd RL. The phenotypic heterogeneity of mouse thymic stromal cells. Immunology. 1990; 70(1):66-74. (Biology). View Reference
  4. Gumley TP, McKenzie IF, Kozak CA, Sandrin MS. Isolation and characterization of cDNA clones for the mouse thymocyte B cell antigen (ThB). J Immunol. 1992; 149(8):2615-2618. (Biology). View Reference
  5. Gumley TP, McKenzie IF, Sandrin MS. Polymorphism at the mouse Thb locus. Immunogenetics. 1994; 39(6):390-394. (Clone-specific: Immunoprecipitation). View Reference
  6. Matossian-Rogers A, Rogers P, Ledbetter JA, Herzenberg LA. Molecular weight determination of two genetically linked cell surface murine antigens: ThB and Ly-6. Immunogenetics. 1982; 15(6):591-599. (Clone-specific: Immunoprecipitation). View Reference
View All (6) View Less
561149 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.