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PE Rat Anti-Mouse CRIg
PE Rat Anti-Mouse CRIg

Two-color flow cytometric analysis of CRIg expression on mouse peritoneal macrophages. Resident peritoneal cells were harvested from BALB/c mice, washed, and then stained with BD Horizon™ BUV395 Rat Anti-Mouse F4/80 antibody (Cat. No. 565614) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Middle Plot) or PE Rat Anti-Mouse CRIg antibody (Cat. No. 566737; Right Plot) at 0.5 µg/test. Two-color flow cytometric dot plots showing the correlated expression of CRIg (or Ig Isotype control staining) versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of viable macrophages (Left Plot). Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Two-color flow cytometric analysis of CRIg expression on mouse peritoneal macrophages. Resident peritoneal cells were harvested from BALB/c mice, washed, and then stained with BD Horizon™ BUV395 Rat Anti-Mouse F4/80 antibody (Cat. No. 565614) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Middle Plot) or PE Rat Anti-Mouse CRIg antibody (Cat. No. 566737; Right Plot) at 0.5 µg/test. Two-color flow cytometric dot plots showing the correlated expression of CRIg (or Ig Isotype control staining) versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of viable macrophages (Left Plot). Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Product Details
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BD Pharmingen™
CRIg; Vsig4; Z39IG
Mouse (QC Testing)
Rat IgG2a, κ
Mouse CRIg Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
278180
AB_2869839
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566737 Rev. 1
Antibody Details
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17C9

The 17C9 monoclonal antibody specifically recognizes Complement receptor of the immunoglobulin superfamily (CRIg) which is encoded by the Vsig4 (V-set and immunoglobulin domain containing 4) gene. CRIg is a ~45 kDa single-pass type I transmembrane glycoprotein that contains a variable (V-type) immunoglobulin domain in its extracellular region which is required for binding complement components and promoting phagocytosis. CRIg is expressed on Kupffer cells and tissue resident macrophages, including a subset of resident peritoneal macrophages. CRIg binds complement fragments C3b and iC3b and plays important roles in innate and adaptive immunity. This complement receptor is involved in regulation of the complement cascade and serves as a phagocytic receptor in the clearance of pathogens, immune complexes, and apoptotic cells. CRIg-positive macrophages can reportedly function in the negative regulation of T-cell proliferation and IL-2 production.

566737 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566737 Rev.1
Citations & References
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Development References (4)

  1. Fu W, Wojtkiewicz G, Weissleder R, Benoist C, Mathis D. Early window of diabetes determinism in NOD mice, dependent on the complement receptor CRIg, identified by noninvasive imaging. Nat Immunol. 2012; 13(4):361-368. (Clone-specific: Flow cytometry). View Reference
  2. Helmy KY, Katschke KJ, Jr., et al. CRIg: a macrophage complement receptor required for phagocytosis of circulating pathogens. Cell. 2006; 124(5):915-927. (Biology). View Reference
  3. Small AG, Al-Baghdadi M, Quach A, Hii C, Ferrante A. Complement receptor immunoglobulin: a control point in infection and immunity, inflammation and cancer. Swiss Med Wkly. 2016; 146:w14301. (Biology). View Reference
  4. Vogt L, Schmitz N, Kurrer MO, et al. VSIG4, a B7 family-related protein, is a negative regulator of T cell activation. J Clin Invest. 2006; 116(10):2817-2826. (Biology). View Reference
View All (4) View Less
566737 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.