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PE Mouse anti-SNAI2/Slug
PE Mouse anti-SNAI2/Slug

Flow cytometric analysis of SNAI2/Slug expression in human rhadomyosarcoma cells. RH-30 cells (DSMZ, ACC 489) were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574). The fixed cells were then stained with either PE Mouse IgG1, κ Isotype Control (Cat No. 554680, dashed line) or PE Mouse anti-SNAI2/Slug (solid line). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSRFortessa™ Cell Analyser.

Flow cytometric analysis of SNAI2/Slug expression in human rhadomyosarcoma cells. RH-30 cells (DSMZ, ACC 489) were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574). The fixed cells were then stained with either PE Mouse IgG1, κ Isotype Control (Cat No. 554680, dashed line) or PE Mouse anti-SNAI2/Slug (solid line). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSRFortessa™ Cell Analyser.

Product Details
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BD Pharmingen™
Snai2; SLUG; Slugh; SLUGH
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG1, κ
Human SNAI2 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2738866
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
564615 Rev. 1
Antibody Details
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S43-1259

The S43-1259 monoclonal antibody specifically recognizes SNAI2, also named Slug, which is a zinc finger transcriptional repressor first recognized for its roles in embryogenesis. SNAI2/Slug and other members of the snail family of transcription factors inhibit the expression of E-cadherin, which plays important roles in cell adhesion and in the maintenance of tissue structure. SNAI2/Slug activation and E-cadherin inhibition induces epithelial-mesenchymal transition (EMT) at several stages of embryonic development and also contributes to the invasiveness of malignancies. Aberrant expression of SNAI2/Slug has also been linked to cancer stem cell formation, cell cycle regulation and apoptosis.  SNAI2/Slug is a downstream effector of various signaling pathways, including Akt, Wnt and SCF/ckit signaling.

564615 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
564615 Rev.1
Citations & References
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Development References (4)

  1. Carpenter RL, Paw I, Dewhirst MW, Lo HW. Akt phosphorylates and activates HSF-1 independent of heat shock, leading to Slug overexpression and epithelial-mesenchymal transition (EMT) of HER2-overexpressing breast cancer cells. Oncogene. 2014; ePub(Biology). View Reference
  2. Francí C, Takkunen M, Dave N, et al. Expression of Snail protein in tumor-stroma interface. Oncogene. 2006; 25(37):5134-5144. (Biology). View Reference
  3. Guo W, Keckesova Z, Donaher JL, et al. Slug and Sox9 cooperatively determine the mammary stem cell state. Cell. 2012; 148(5):1015-1028. (Biology). View Reference
  4. Hay ED, Zuk A. Transformations between epithelium and mesenchyme: normal, pathological, and experimentally induced. Am J Kidney Dis. 1995; 26(4):678-690. (Biology). View Reference
View All (4) View Less
564615 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.