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PE Mouse Anti-Rat CD314 (NKG2-D)
PE Mouse Anti-Rat CD314 (NKG2-D)

Two-color flow cytometric analysis of CD314 (NKG2-D) expression on rat splenic leukocytes.  Lewis rat splenic leucocytes were stained with FITC Mouse Anti-Rat CD161a antibody (Cat. No. 555008/ 561781) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680, Left Panel) or PE Mouse Anti-Rat CD314 (NKG2-D) antibody (Cat. No. 563263; Right Panel).  The two-color flow cytometric dot plots show the correlated expression patterns of CD314 (or Ig Isotype control staining) versus CD161 for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

Two-color flow cytometric analysis of CD314 (NKG2-D) expression on rat splenic leukocytes.  Lewis rat splenic leucocytes were stained with FITC Mouse Anti-Rat CD161a antibody (Cat. No. 555008/ 561781) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680, Left Panel) or PE Mouse Anti-Rat CD314 (NKG2-D) antibody (Cat. No. 563263; Right Panel).  The two-color flow cytometric dot plots show the correlated expression patterns of CD314 (or Ig Isotype control staining) versus CD161 for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

Product Details
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BD Pharmingen™
NKR-P2, Nkg2d, Nkrp2, Klrk1; NKLLR
Rat (QC Testing)
Mouse IgG1, κ
Rat NKG2-D Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738103
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
563263 Rev. 1
Antibody Details
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9C11G4

The 9C11G4 monoclonal antibody specifically binds to CD314, also known as NKG2-D, NKR-P2 and NKLLR. CD314 is encoded by Klrk1 (Killer cell lectin-like receptor subfamily K, member 1). It is a type II transmembrane glycoprotein of the C-type lectin family. This lectin-like receptor is expressed by several cell types including NK cells, NKT cells, γδ T lymphocytes and CD8-positive αβ T lymphocytes. CD314 acts as an activating receptor that can induce NK effector cell activities. NK cells can reportedly bind to members of the RAE1 family, including cell surface RAE-1L and RRLT as ligands, through their CD314 receptors. NK cells are thereby induced to secrete IFN-γ and exert cytotoxicity that may play a role in allograft rejection.

563263 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
563263 Rev.1
Citations & References
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Development References (4)

  1. Berg SF, Dissen E, Westgaard IH, Fossum S. Molecular characterization of rat NKR-P2, a lectin-like receptor expressed by NK cells and resting T cells. Int Immunol. 1998; 10(4):379-385. (Biology). View Reference
  2. Savithri B, Khar A. A transmembrane-anchored rat RAE-1-like transcript as a ligand for NKR-P2, the rat ortholog of human and mouse NKG2D. Eur J Immunol. 2006; 36(1):107-117. (Biology). View Reference
  3. Wai LE, Garcia JA, Martinez OM, Krams SM. Distinct roles for the NK cell-activating receptors in mediating interactions with dendritic cells and tumor cells. J Immunol. 2011; 186(1):222-229. (Biology). View Reference
  4. Zhuo M, Fujiki M, Wang M, et al. Identification of the rat NKG2D ligands, RAE1L and RRLT, and their role in allograft rejection. Eur J Immunol. 2010; 40(6):1748-1757. (Biology). View Reference
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563263 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.