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PE Mouse Anti-Rat CD26
Product Details
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BD Pharmingen™
Dpp4; dipeptidyl peptidase IV; DPPIV; Cd26
Rat (QC Testing)
Mouse BALB/c IgG2a, κ
Rat dendritic cells enriched from thoracic duct lymph
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_397294
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
559641 Rev. 5
Antibody Details
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OX-61

The OX-61 monoclonal antibody specifically recognizes a type II transmembrane glycoprotein, CD26, which is a serine exoprotease identified as dipeptidyl/peptidase IV. Among various biological activities, rat CD26 binds fibronectin and collagen. Rat CD26 is involved in the costimulation of thymocyte proliferation in vitro, particularly the CD4-/CD8- subset, and is developmentally regulated on hematopoietic cells. Although mouse and human CD26 anchor ADA (adenosine deaminase) to cell membranes, rat CD26 does not function as an ADA-binding protein. Rat CD26 is expressed in lung endothelial cells, as well as in various epithelial cells. T cells express lower levels of CD26 than CD4-/CD8- thymocytes. The distribution of CD26 antigen in rat bone marrow cells is similar to that of human CD26. OX-61 monoclonal antibody stains CD4+, CD8+, and Ig+ lymphocytes, and the staining increases upon activation.

559641 Rev. 5
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
559641 Rev.5
Citations & References
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Development References (8)

  1. Bristol LA, Sakaguchi K, Appella E, Doyle D, Takacs L. Thymocyte costimulating antigen is CD26 (dipeptidyl-peptidase IV). Costimulation of granulocyte, macrophage, and T lineage cell proliferation via CD26. J Immunol. 1992; 149(2):367-372. (Biology). View Reference
  2. Gorrell MD, Wickson J, McCaughan GW. Expression of the rat CD26 antigen (dipeptidyl peptidase IV) on subpopulations of rat lymphocytes. Cell Immunol. 1991; 134(1):205-215. (Clone-specific: Immunohistochemistry, Immunoprecipitation). View Reference
  3. Hanski C, Huhle T, Gossrau R, Reutter W. Direct evidence for the binding of rat liver DPP IV to collagen in vitro. Exp Cell Res. 1988; 178(1):64-72. (Biology). View Reference
  4. Iwaki-Egawa S, Watanabe Y, Fujimoto Y. CD26/dipeptidyl peptidase IV does not work as an adenosine deaminase-binding protein in rat cells. Cell Immunol. 1997; 178(2):180-186. (Biology). View Reference
  5. Johnson RC, Zhu D, Augustin-Voss HG, Pauli BU. Lung endothelial dipeptidyl peptidase IV is an adhesion molecule for lung-metastatic rat breast and prostate carcinoma cells. J Cell Biol. 1993; 121(6):1423-1432. (Biology). View Reference
  6. Kameoka J, Tanaka T, Nojima Y, Schlossman SF, Morimoto C. Direct association of adenosine deaminase with a T cell activation antigen, CD26. Science. 1993; 261(5120):466-469. (Biology). View Reference
  7. McCaughan GW, Wickson JE, Creswick PF, Gorrell MD. Identification of the bile canalicular cell surface molecule GP110 as the ectopeptidase dipeptidyl peptidase IV: an analysis by tissue distribution, purification and N-terminal amino acid sequence. Hepatology. 1990; 11(4):534-544. (Immunogen: Immunohistochemistry, Immunoprecipitation, Western blot). View Reference
  8. Piazza GA, Callanan HM, Mowery J, Hixson DC. Evidence for a role of dipeptidyl peptidase IV in fibronectin-mediated interactions of hepatocytes with extracellular matrix. Biochem J. 1989; 262(1):327-334. (Biology). View Reference
View All (8) View Less
559641 Rev. 5

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.