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PE Mouse Anti-Rat CD1d
PE Mouse Anti-Rat CD1d

Two-color flow cytometric analysis of CD1d expression on rat thymocytes and splenocytes. Rat thymocytes and splenic leucocytes were stained with APC Mouse Anti-Rat CD3 antibody (Cat. No. 557030) and either PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648) or PE Mouse Anti-Rat CD1d antibody (Cat. No. 564634). Two-color flow cytometric contour plots showing the correlated expression of CD1d (or Ig Isotype control staining) versus CD3 were derived from gated events with the forward and side-light scatter characteristics of viable thymocytes (Left Panel) or splenic leucocytes (Right Panel). Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Two-color flow cytometric analysis of CD1d expression on rat thymocytes and splenocytes. Rat thymocytes and splenic leucocytes were stained with APC Mouse Anti-Rat CD3 antibody (Cat. No. 557030) and either PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648) or PE Mouse Anti-Rat CD1d antibody (Cat. No. 564634). Two-color flow cytometric contour plots showing the correlated expression of CD1d (or Ig Isotype control staining) versus CD3 were derived from gated events with the forward and side-light scatter characteristics of viable thymocytes (Left Panel) or splenic leucocytes (Right Panel). Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Product Details
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BD Pharmingen™
Cd1d; Antigen-presenting glycoprotein CD1d; CD1d1
Rat (QC Testing), Mouse (Tested in Development)
Mouse BALB/c IgG2a, κ
Rat CD1d Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738875
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
564634 Rev. 1
Antibody Details
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WTH2

The WTH2 monoclonal antibody specifically binds to rat CD1d and crossreacts with mouse CD1d. CD1d is a type I transmembrane glycoprotein that is noncovalently associated with β2-microglobulin. CD1d is expressed on various cell types including subsets of thymocytes, T cells, B cells, monocytes, macrophages, dendritic cells, endothelial cells and epithelial cells. Although structurally similar to MHC Class I antigens, CD1d molecules are rather non-polymorphic and serve to present non-peptide antigens such as endogenous or microbial glycolipids to T lymphocytes (NKT cells). The WTH1 monoclonal antibody reportedly binds to a non-overlapping epitope on rat CD1d and also crossreacts with mouse CD1d when compared with WTH2. Both WTH1 and WTH2 reportedly interfere with antigen recognition by CD1d-restricted T cells.

564634 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
564634 Rev.1
Citations & References
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Development References (3)

  1. Ichimiya S, Kikuchi K, Matsuura A. Structural analysis of the rat homologue of CD1. Evidence for evolutionary conservation of the CD1D class and widespread transcription by rat cells. J Immunol. 1994; 153(3):1112-1123. (Biology). View Reference
  2. Katabami S, Matsuura A, Chen HZ, Imai K, Kikuchi K. Structural organization of rat CD1 typifies evolutionarily conserved CD1D class genes. Immunogenetics. 1998; 48(1):22-31. (Biology). View Reference
  3. Monzon-Casanova E, Steiniger B, Schweigle S, et al. CD1d expression in paneth cells and rat exocrine pancreas revealed by novel monoclonal antibodies which differentially affect NKT cell activation. PLoS ONE. 2010; 5(9):e13089. (Immunogen: Blocking, ELISA, Flow cytometry, Functional assay, Immunohistochemistry, Immunoprecipitation, Inhibition, Western blot). View Reference
564634 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.