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PE Mouse Anti-Human IRF8
PE Mouse Anti-Human IRF8
Multicolor flow cytometric analysis of IRF8 expression in human peripheral blood mononuclear cells (PBMCs). PBMCs were stained with FITC Mouse Anti-Human CD19 antibody (Cat. No. 555412/560994; Top Plots) and BD Horizon™ BV421 Mouse Anti-Human CD123 (Cat. No. 562517/565928; Bottom Plots) antibodies. They were then fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) followed by staining with either PE Mouse IgG1, κ Isotype Control (Cat. No. 559320; Left Plots) or PE Mouse Anti-Human IRF8 antibody (Cat. No. 566373; Right Plots) at 0.06 μg/test. Two-color flow cytometric contour plots showing the correlated expression of CD19 or CD123 versus IRF8 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Multicolor flow cytometric analysis of IRF8 expression in human peripheral blood mononuclear cells (PBMCs). PBMCs were stained with FITC Mouse Anti-Human CD19 antibody (Cat. No. 555412/560994; Top Plots) and BD Horizon™ BV421 Mouse Anti-Human CD123 (Cat. No. 562517/565928; Bottom Plots) antibodies. They were then fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) followed by staining with either PE Mouse IgG1, κ Isotype Control (Cat. No. 559320; Left Plots) or PE Mouse Anti-Human IRF8 antibody (Cat. No. 566373; Right Plots) at 0.06 μg/test. Two-color flow cytometric contour plots showing the correlated expression of CD19 or CD123 versus IRF8 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
IRF-8; IRF8; ICSBP; H-ICSBP; ICSBP1
Human (QC Testing)
Mouse IgG1, κ
Human IRF8 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739716
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566373 Rev. 1
Antibody Details
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U31-644

The U31-644 monoclonal antibody specifically recognizes human Interferon regulatory factor 8 (IRF8) which is also known as Interferon consensus sequence-binding protein (ICSBP or H-ICSBP). IRF8 is a ~50 kDa transcription factor that belongs to the interferon (IFN) regulatory factor (IRF) family which consists of 9 members in both humans and mice. IRF8 is variably expressed in hematopoietic cells including monocytes, macrophages, dendritic cells, and B cells. IRF8 has a conserved N-terminal DNA-binding domain (DBD) and a divergent C-terminal region that functions as the regulatory domain which specifies interactions between IRF8 and other IRF family members, transcription factors, or cofactors. IRF8 functions as a transcriptional activator or repressor depending on the formation of heterodimeric DNA binding complexes with these different partners. IRF8 binds to specific DNA motifs including IFN-stimulated response elements (ISRE) and regulates expression of genes including those that encode IFN-alpha (IFN-α), IFN-beta (IFN-β), and IFN-inducible MHC class I genes. IRF8 plays an important in the development of myeloid cells and B cells. It functions in innate and adaptive immunity by regulating the effector functions of monocytes/macrophages, B cells and dendritic cell subsets. IRF8 participates in the induction of Type I IFNs, TNF, IL-6, and IL-12 expression by leucocytes stimulated with ligands for Toll-like receptors and other pattern recognition receptors (PRRs). IRF8 is also involved in cell cycle regulations and apoptosis and may play a role in tumorigenesis.

        

566373 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566373 Rev.1
Citations & References
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View product citations for antibody "566373" on CiteAb

Development References (9)

  1. Aliberti J, Schulz O, Pennington DJ, et al. Essential role for ICSBP in the in vivo development of murine CD8alpha + dendritic cells.. Blood. 2003; 101(1):305-10. (Biology). View Reference
  2. Driggers PH, Ennist DL, Gleason SL, et al. An interferon gamma-regulated protein that binds the interferon-inducible enhancer element of major histocompatibility complex class I genes.. Proc Natl Acad Sci USA. 1990; 87(10):3743-7. (Biology). View Reference
  3. Hambleton S, Salem S, Bustamante J, et al. IRF8 mutations and human dendritic-cell immunodeficiency.. N Engl J Med. 2011; 365(2):127-38. (Biology). View Reference
  4. Ikushima H, Negishi H, Taniguchi T. The IRF family transcription factors at the interface of innate and adaptive immune responses.. Cold Spring Harb Symp Quant Biol. 2013; 78:105-16. (Biology). View Reference
  5. Nündel K, Green NM, Shaffer AL, et al. Cell-intrinsic expression of TLR9 in autoreactive B cells constrains BCR/TLR7-dependent responses.. J Immunol. 2015; 194(6):2504-12. (Biology). View Reference
  6. Tsujimura H, Tamura T, Ozato K. Cutting edge: IFN consensus sequence binding protein/IFN regulatory factor 8 drives the development of type I IFN-producing plasmacytoid dendritic cells.. J Immunol. 2003; 170(3):1131-5. (Biology). View Reference
  7. Wang H, Morse HC. IRF8 regulates myeloid and B lymphoid lineage diversification.. Immunol Res. 2009; 43(1-3):109-17. (Biology). View Reference
  8. Weisz A, Marx P, Sharf R, et al. Human interferon consensus sequence binding protein is a negative regulator of enhancer elements common to interferon-inducible genes.. J Biol Chem. 1992; 267(35):25589-96. (Biology). View Reference
  9. Yanai H, Negishi H, Taniguchi T. The IRF family of transcription factors: Inception, impact and implications in oncogenesis.. Oncoimmunology. 2012; 1(8):1376-1386. (Biology). View Reference
View All (9) View Less
566373 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.