BD Pharmingen™ FITC Rat Anti-Human B7-H6
Clone 1A5 (RUO)
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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
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The 1A5 monoclonal antibody specifically recognizes B7 homolog 6 (B7-H6). B7-H6 is a ~51 kDa type I transmembrane glycoprotein that is encoded by NCR3LG1 (natural killer cell cytotoxicity receptor 3 ligand 1) which belongs to the B7 family of immunoreceptors. B7-H6 contains one IgV-like and one IgC1-like domain in its extracellular region followed by a transmembrane region and an intracytoplasmic region with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an SH2- and SH3-binding motifs. Although B7-H6 is not expressed by normal cells from healthy individuals, it is variably expressed on stressed cells including tumor cells and tumor cell lines, and can be moderately expressed on proinflammatory monocytes and neutrophils. Binding of B7-H6 to CD337 (NKp30/NCR3) induces NK cell activation, cytotoxicity, and cytokine secretion which suggests it may play an important role in immunosurveillance against certain tumors and infectious diseases.
Development References (3)
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Brandt CS, Baratin M, Yi EC, et al. The B7 family member B7-H6 is a tumor cell ligand for the activating natural killer cell receptor NKp30 in humans.. J Exp Med. 2009; 206(7):1495-503. (Biology). View Reference
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Matta J, Baratin M, Chiche L, et al. Induction of B7-H6, a ligand for the natural killer cell-activating receptor NKp30, in inflammatory conditions.. Blood. 2013; 122(3):394-404. (Biology). View Reference
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Wang R, Jaw JJ, Stutzman NC, Zou Z, Sun PD. Natural killer cell-produced IFN-γ and TNF-α induce target cell cytolysis through up-regulation of ICAM-1.. J Leukoc Biol. 2012; 91(2):299-309. (Clone-specific: Flow cytometry). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.