The OX-34 antibody reacts with CD2 (LFA-2), a member of the immunoglobulin superfamily. In the rat, CD2 is expressed on thymocytes, T lymphocytes in spleen and lymph node, dendritic epidermal T cells, splenic macrophages, and NK cells, but not on B cells, most intestinal intraepithelial lymphocytes, or peritoneal and liver macrophages. CD2 can associate with the T-cell receptor complex, and it may function in both intercellular adhesion and signal transduction. In the rat, CD48 and CD59 have been identified as ligands for CD2. OX-34 mAb binds to the extracellular portion of CD2, and it blocks the binding of CD2 to CD48. While OX-34 antibody does not activate T cells, it partially blocks activation by immobilized mAbs to CD3 (clone G4.18) and αβ T-cell receptor (clone R73), and it partially inhibits allogeneic mixed lymphocyte reactions. Moreover, in vivo administration of OX-34 antibody depletes peripheral T cells and prevents allograft rejection.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.