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BV421 Mouse Anti-Human CD84
BV421 Mouse Anti-Human CD84

Flow cytometric analysis of CD84 expression on human peripheral blood leucocytes. Whole blood was stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; Left Plot) or BD Horizon BV421 Mouse Anti-Human CD84 antibody (Cat. No. 566094; Right Plot). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Flow cytometric plots showing the correlated expression of side scattered-light signals (SSC) versus CD84 (or Ig Isotype Control staining) were derived from gated events with the forward and side light-scatter characteristics of intact human peripheral blood leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.

BV421 Mouse Anti-Human CD84

Two-color flow cytometric analysis of CD84 expression on human peripheral blood lymphocytes. Whole blood was stained with PE CD19 (Cat. No. 555413/561741) and either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; Left Plot) or BD Horizon BV421 Mouse Anti-Human CD84 antibody (Cat. No. 566094; Right Plot). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Two-parameter flow cytometric plots showing the correlated expression of CD19 versus CD84 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact human peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.

Flow cytometric analysis of CD84 expression on human peripheral blood leucocytes. Whole blood was stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; Left Plot) or BD Horizon BV421 Mouse Anti-Human CD84 antibody (Cat. No. 566094; Right Plot). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Flow cytometric plots showing the correlated expression of side scattered-light signals (SSC) versus CD84 (or Ig Isotype Control staining) were derived from gated events with the forward and side light-scatter characteristics of intact human peripheral blood leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.

Two-color flow cytometric analysis of CD84 expression on human peripheral blood lymphocytes. Whole blood was stained with PE CD19 (Cat. No. 555413/561741) and either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; Left Plot) or BD Horizon BV421 Mouse Anti-Human CD84 antibody (Cat. No. 566094; Right Plot). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Two-parameter flow cytometric plots showing the correlated expression of CD19 versus CD84 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact human peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.

Product Details
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BD Horizon™
hCD84; SLAMF5; SLAF5; LY9B; Hly9-beta; Cell surface antigen MAX.3
Human (QC Testing)
Mouse IgG1, κ
Namalwa Cell Line
Flow cytometry (Routinely Tested)
5 µl
V B057; VI B082
AB_2739498
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566094 Rev. 1
Antibody Details
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2G7

The 2G7 monoclonal antibody specifically binds to CD84, which is also known as SLAM family member 5 (SLAMF5), Hly9-beta, LY9B, or Cell surface antigen MAX.3. CD84 is 64-82 kDa type I transmembrane glycoprotein that belongs to the CD2 family within the Ig gene superfamily. CD84 is expressed on mature B cells, thymocytes, CD45RO+ T cells, NK cells, monocytes, and platelets. CD84 is also strongly expressed by tissue macrophages. CD84 may play a role as a homotypic adhesion molecule that mediates leucocyte interactions and signaling.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

566094 Rev. 1
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
566094 Rev.1
Citations & References
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Development References (9)

  1. Engel P, Smith H, Tedder TF. Phenotypic analysis with the B-cell Unknown Panel mAb. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:607-620.
  2. Engel P, Wagner N, Tedder TF. CDw84 Workshop Report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:699-700.
  3. Martin M, Romero X, de la Fuente MA, et al. CD84 functions as a homophilic adhesion molecule and enhances IFN-gamma secretion: adhesion is mediated by Ig-like domain 1. J Immunol. 2001; 167(7):3668-3676. (Biology). View Reference
  4. Romero X, Benítez D, March S, Vilella R, Miralpeix M, Engel P. Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4). Tissue Antigens. 2004; 64(2):132-144. (Biology). View Reference
  5. Romero X, de la Fuente MA, Tovar V, et al. Characterization of a novel panel of CD84 monoclonal antibodies. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:103-104.
  6. Sayós J, Martín M, Chen A, et al. Cell surface receptors Ly-9 and CD84 recruit the X-linked lymphoproliferative disease gene product SAP. Blood. 2001; 97(12):3867-3874. (Biology). View Reference
  7. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
  8. de la Fuente MA, Pizcueta P, Engel P. CD84 Workshop Panel report. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:193-194.
  9. de la Fuente MA, Pizcueta P, Nadal M, Bosch J, Engel P. CD84 leukocyte antigen is a new member of the Ig superfamily. Blood. 1997; 90(6):2398-2405. (Clone-specific: Flow cytometry, Immunoprecipitation). View Reference
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566094 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.