The A8 monoclonal antibody specifically binds to CD100 which is also known as Semaphorin-4D (SEMA4D). CD100 is a 150 kDa type I transmembrane glycoprotein that belongs to the semaphorin family. CD100 is widely expressed in the nervous and immune systems. Although it is expressed on T cells, its expression is significantly upregulated on activated T cells. It is also expressed on NK cells, B cells, and monocytes. CD100 is an adhesion molecule that functions as a signaling molecule which plays a role in lymphocyte activation. CD100 interaction with CD72 enhances B cell signaling and ultimately promotes B cell activation and survival.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.