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BV421 Hamster Anti-Mouse CD209b (SIGN-R1)
BV421 Hamster Anti-Mouse CD209b (SIGN-R1)
Multiparameter flow cytometric analysis of CD209b (SIGN-R1) expression on BALB/c versus C57BL/6 Mouse Peritoneal Exudate Cells (PECs). Resident peritoneal cells were harvested from BALB/c (Top Plots) or C57BL/6 (Bottom Plots) Mice and were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553142). The cells were stained with Alexa Fluor™ 647 Rat Anti-CD11b antibody (Cat. No. 557686) and with either BD Horizon™ BV421 Hamster Isotype Control (Cat. No. 562612, Left Plot) or BD OptiBuild™ BV421 Hamster Anti-Mouse CD209b (SIGN-R1) antibody (Cat. No. 756157, Right Plot) at 0.25 µg/test. A bivariate pseudocolor density plot showing the correlated expression of CD209b (SIGN-R1) [or Ig Isotype Control Staining] versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Multiparameter flow cytometric analysis of CD209b (SIGN-R1) expression on BALB/c versus C57BL/6 Mouse Peritoneal Exudate Cells (PECs). Resident peritoneal cells were harvested from BALB/c (Top Plots) or C57BL/6 (Bottom Plots) Mice and were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553142). The cells were stained with Alexa Fluor™ 647 Rat Anti-CD11b antibody (Cat. No. 557686) and with either BD Horizon™ BV421 Hamster Isotype Control (Cat. No. 562612, Left Plot) or BD OptiBuild™ BV421 Hamster Anti-Mouse CD209b (SIGN-R1) antibody (Cat. No. 756157, Right Plot) at 0.25 µg/test. A bivariate pseudocolor density plot showing the correlated expression of CD209b (SIGN-R1) [or Ig Isotype Control Staining] versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD OptiBuild™
Cd209b; DC-SIGNR1; mSIGNR1
Mouse (Tested in Development)
Armenian Hamster IgG, κ
Recombinant Mouse SIGN-R1 ECD–Mouse IgG-Fc Protein
Flow cytometry (Qualified)
0.2 mg/ml
69165
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

       BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

       For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  6. Researchers should determine the optimal concentration of this reagent for their individual applications.
  7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  10. For U.S. patents that may apply, see bd.com/patents.
756157 Rev. 3
Antibody Details
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22D1

The 22D1 monoclonal antibody specifically recognizes mouse CD209 antigen-like protein B (CD209b) which is also known as Mouse SIGN-related 1 (mSIGNR1 or SIGN-R1). CD209b is a mouse homolog of human CD209 (DC-SIGN) and is also referred to as DC-SIGN-related protein 1 (DC-SIGNR1). CD209b is an ~37 kDa type II transmembrane glycoprotein that is encoded by Cd209b (CD209b antigen) which belongs to the C-type lectin family. It contains a single C-terminal C-type lectin domain and is involved in the recognition and internalization of microbial polysaccharides. CD209b is expressed on macrophages residing in the splenic marginal zone, the medullary and trabecular sinuses of lymph nodes, and peritoneal cavity. CD209b is involved in the clearance and innate immune responses to microbial molecules including polysaccharides and lipopolysaccharides (LPS) and the uptake of encapsulated microbes. It may also affect leucocyte migration through its adhesive interaction with CD54 (ICAM-2).

756157 Rev. 3
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
756157 Rev.3
Citations & References
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View product citations for antibody "756157" on CiteAb

Development References (4)

  1. Camara A, Lavanant AC, Abe J, et al. CD169+ macrophages in lymph node and spleen critically depend on dual RANK and LTbetaR signaling.. Proc Natl Acad Sci U S A. 2022; 119(3):e2108540119. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
  2. Kang YS, Kim JY, Bruening SA, et al. The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen.. Proc Natl Acad Sci USA. 2004; 101(1):215-20. (Immunogen: Flow cytometry, Fluorescence microscopy, In vivo exacerbation, Western blot). View Reference
  3. Kawauchi Y, Igarashi M, Kojima N. C-type lectin receptor SIGNR1 expressed on peritoneal phagocytic cells with an immature dendritic cell-like phenotype is involved in uptake of oligomannose-coated liposomes and subsequent cell maturation.. Cell Immunol. 2014; 287(2):121-8. (Clone-specific: Flow cytometry). View Reference
  4. Taylor PR, Martinez-Pomares L, Stacey M, Lin HH, Brown GD, Gordon S. Macrophage receptors and immune recognition.. Annu Rev Immunol. 2005; 23:901-44. (Clone-specific: In vivo exacerbation). View Reference
View All (4) View Less
756157 Rev. 3

 

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