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Biotin Rat anti-Mouse CD209a
Biotin Rat anti-Mouse CD209a

Two-color analysis of CD209a expression on peritoneal DC. After preincubation with Mouse BD Fc Block™ purified mAb 2.4G2 (Cat. no. 553141/553142), BALB/c resident peritoneal cells were stained with APC anti-mouse CD11c mAb HL3 (Cat. no. 550261) and either Biotin Rat IgG2a, κ isotype control mAb R35-95 (Cat. no. 553928, left panel) or Biotin mAb 5H10/CIRE (right panel), followed by Streptavidin-Phycoerythrin (SAv-PE). Dead cells were excluded by staining with propidium iodide (Cat. no. 556463). Almost all of the CD209a-expressing cells are CD11c-positive DC. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Two-color analysis of CD209a expression on peritoneal DC. After preincubation with Mouse BD Fc Block™ purified mAb 2.4G2 (Cat. no. 553141/553142), BALB/c resident peritoneal cells were stained with APC anti-mouse CD11c mAb HL3 (Cat. no. 550261) and either Biotin Rat IgG2a, κ isotype control mAb R35-95 (Cat. no. 553928, left panel) or Biotin mAb 5H10/CIRE (right panel), followed by Streptavidin-Phycoerythrin (SAv-PE). Dead cells were excluded by staining with propidium iodide (Cat. no. 556463). Almost all of the CD209a-expressing cells are CD11c-positive DC. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Product Details
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BD Pharmingen™
CD209; Cire; DC-SIGN; Dcsign; DC-SIGN1; CDSIGN
Mouse (QC Testing)
Rat IgG2a, κ
Peptide Derived from Extracellular Region of CD209a/CIRE
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_397012
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. An isotype control should be used at the same concentration as the antibody of interest.
558073 Rev. 3
Antibody Details
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5H10

The 5H10 monoclonal antibody specifically binds to mouse CD209a, or CIRE, which is the most closely related homolog of human DC-SIGN. In the mouse, several homologs of human CD209 (DC-SIGN) have been described. CD209a is a 238-amino acid type-II transmembrane C-type lectin, with an extracellular carbohydrate recognition domain. It can bind to mannosylated ligands in a Ca++ dependent manner. CD209a is expressed by some conventional CD4+ and CD4-CD8- dendritic cells (DC) but not by CD8+ DC. It is also expressed on monocytes, splenic plasmacytoid pre-DC, macrophages, and resident peritoneal cells. CD209a expression is downregulated upon cellular activation. CD209a is thought to play a role in the internalization and presentation of antigens to initiate immune responses.

558073 Rev. 3
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
558073 Rev.3
Citations & References
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Development References (5)

  1. Baribaud F, Pohlmann S, Sparwasser T, et al. Functional and antigenic characterization of human, rhesus macaque pigtailed macaque, and murine DC-SIGN. J Virol. 2001; 75(21):10281-10289. (Biology). View Reference
  2. Caminschi I, Lucas KM, O'Keeffe MA, et al. Molecular cloning of a C-type lectin superfamily protein differentially expressed by CD8alpha(-) splenic dendritic cells. Mol Immunol. 2001; 38(5):365-373. (Biology). View Reference
  3. O'Keeffe M, Hochrein H, Vremec D, et al. Mouse plasmacytoid cells: long-lived cells, heterogeneous in surface phenotype and function, that differentiate into CD8(+) dendritic cells only after microbial stimulus. J Exp Med. 2002; 196(10):1307-1319. (Clone-specific: Flow cytometry). View Reference
  4. Parent SA, Zhang T, Chrebet G, et al. Molecular characterization of the murine SIGNR1 gene encoding a C-type lectin homologous to human DC-SIGN and DC-SIGNR. Gene. 2002; 293(1):33. (Biology). View Reference
  5. Park CG, Takahara K, Umemoto E, et al. Five mouse homologues of the human dendritic cell C-type lectin, DC-SIGN. Int Immunol. 2001; 13(10):1283-1290. (Biology). View Reference
View All (5) View Less
558073 Rev. 3

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.