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Biotin Mouse Anti-Mouse CD244.2
Biotin Mouse Anti-Mouse CD244.2

Two-color analysis of CD244.2 expression on LAK cells. C57BL/6 LAK cells were simultaneously stained with PE-conjugated PK136 (anti-mouse NK1.1, Cat. No. 557391/553165, both panels) and biotinylated 2B4 (right panel) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057, both panels). Flow cytometry was performed on a BD FACScan™ System (BD Biosciences, San Jose, CA).

Two-color analysis of CD244.2 expression on LAK cells. C57BL/6 LAK cells were simultaneously stained with PE-conjugated PK136 (anti-mouse NK1.1, Cat. No. 557391/553165, both panels) and biotinylated 2B4 (right panel) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057, both panels). Flow cytometry was performed on a BD FACScan™ System (BD Biosciences, San Jose, CA).

Product Details
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BD Pharmingen™
CD242.2; 2B4 B6 Alloantigen; Ly90; NAIL; Nmrk; SLAMF4
Mouse (QC Testing)
Mouse 129, also known as 129/J or 129/SvJ IgG2b, κ
rIL-2-propagated NK1.1+ cells from C57BL/6 mice
Flow cytometry (Routinely Tested)
0.5 mg/ml
18106
AB_396714
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
557452 Rev. 1
Antibody Details
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2B4

CD244 is a type I transmembrane glycoprotein that is also known as Natural killer cell receptor 2B4 (NKR2B4), or Signaling lymphocytic activation molecule 4 (SLAMF4). CD244 is a member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF). It is expressed on all natural killer (NK) cells, IL-2-activated NK (LAK) cells, committed progenitors of NK cells, and a subset of T lymphocytes which mediate non-MHC-restricted cytoxicity, including dendritic epidermal T cells. The 2B4 monoclonal antibody specifically binds to CD244.2, the 2B4 alloantigen which is expressed on C57BL/6 and C58/J mice, but not in most strains tested (A/J, AKR, BALB/c, CBA/J, CBA/N, C3H/He, C57BR, DBA/1, DBA/2, NZB, SJL/J, 129). At least two isoforms of CD244.2 protein, products of alternative splicing of hnRNA, are expressed on IL-2-activated C57BL/6 NK cells. They differ only in their cytoplasmic domains; with 2B4L (150-aa cytoplasmic tail) having inhibitory activity and 2B4S (93-aa tail) being stimulatory. The extracellular domain of CD244 is a ligand for another CD2 IgSF member, CD48. 2B4 antibody activates lytic and secretory functions of IL-2-cultured NK cells and CD244.2+ T cells.

557452 Rev. 1
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
557452 Rev.1
Citations & References
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Development References (13)

  1. Brown MH, Boles K, van der Merwe PA, Kumar V, Mathew PA, Barclay AN. 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. J Exp Med. 1998; 188(11):2083-2090. (Biology). View Reference
  2. Davis SJ, van der Merwe PA. The structure and ligand interactions of CD2: implications for T-cell function. Immunol Today. 1996; 17(4):177-187. (Biology). View Reference
  3. Garni-Wagner BA, Purohit A, Mathew PA, Bennett M, Kumar V. A novel function-associated molecule related to non-MHC-restricted cytotoxicity mediated by activated natural killer cells and T cells. J Immunol. 1993; 151(1):60-70. (Biology). View Reference
  4. Kubota K, Katoh H, Muguruma K, Koyama K. Characterization of a surface membrane molecule expressed by natural killer cells in most inbred mouse strains: monoclonal antibody C9.1 identifies an allelic form of the 2B4 antigen. Immunology. 1999; 96(3):491-497. (Biology). View Reference
  5. Kumaresan PR, Stepp SE, Bennett M, Kumar V, Mathew PA. Molecular cloning of transmembrane and soluble forms of a novel rat natural killer cell receptor related to 2B4. Immunogenetics. 2000; 51(4-5):306-313. (Biology). View Reference
  6. Latchman Y, McKay PF, Reiser H. Identification of the 2B4 molecule as a counter-receptor for CD48. J Immunol. 1998; 161(11):5809-5812. (Biology). View Reference
  7. Mathew PA, Garni-Wagner BA, Land K, et al. Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells. J Immunol. 1993; 151(10):5328-5337. (Biology). View Reference
  8. Rosmaraki EE, Douagi I, Roth C, Colucci F, Cumano A, Di Santo JP. Identification of committed NK cell progenitors in adult murine bone marrow. Eur J Immunol. 2001; 31(6):1900-1909. (Biology). View Reference
  9. Schatzle JD, Sheu S, Stepp SE, Mathew PA, Bennett M, Kumar V. Characterization of inhibitory and stimulatory forms of the murine natural killer cell receptor 2B4. Proc Natl Acad Sci U S A. 1999; 96(7):3870-3875. (Biology). View Reference
  10. Schuhmachers G, Ariizumi K, Mathew PA, Bennett M, Kumar V, Takashima A. 2B4, a new member of the immunoglobulin gene superfamily, is expressed on murine dendritic epidermal T cells and plays a functional role in their killing of skin tumors. J Invest Dermatol. 1995; 105(4):592-596. (Biology). View Reference
  11. Schuhmachers G, Ariizumi K, Mathew PA, Bennett M, Kumar V, Takashima A. Activation of murine epidermal gamma delta T cells through surface 2B4. Eur J Immunol. 1995; 25(4):1117-1120. (Biology). View Reference
  12. Sentman CL, Hackett J Jr, Moore TA, Tutt MM, Bennett M, Kumar V. Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen. Hybridoma. 1989; 8(6):605-614. (Immunogen). View Reference
  13. Stepp SE, Schatzle JD, Bennett M, Kumar V, Mathew PA. Gene structure of the murine NK cell receptor 2B4: presence of two alternatively spliced isoforms with distinct cytoplasmic domains. Eur J Immunol. 1999; 29(8):2392-2399. (Biology). View Reference
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557452 Rev. 1

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