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APC Mouse anti-Human ILT7 (CD85g)
APC Mouse anti-Human ILT7 (CD85g)

Multicolor flow cytometric analysis of ILT7 expressed on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells (PBMC) were stained with FITC Lineage Cocktail 1 (lin 1) (CD3, CD14, CD16, CD19, CD20, CD56; Cat. No. 340546),  PerCP-Cy™5.5 Mouse Anti-Human CD123 (Cat. No. 558714) and either APC Mouse IgG1 κ Isotype Control (Cat. No. 550854; Left Panel) or APC Mouse Anti-Human ILT7 (CD85g) antibody (Cat. No. 562355; Right Panel). The two-color flow cytometric dot plots show ILT7 (or Ig isotype control staining) expression patterns of Lin-1-negative, CD123-positive cells with the forward and side-light scattering characteristics of viable PBMC. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Multicolor flow cytometric analysis of ILT7 expressed on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells (PBMC) were stained with FITC Lineage Cocktail 1 (lin 1) (CD3, CD14, CD16, CD19, CD20, CD56; Cat. No. 340546),  PerCP-Cy™5.5 Mouse Anti-Human CD123 (Cat. No. 558714) and either APC Mouse IgG1 κ Isotype Control (Cat. No. 550854; Left Panel) or APC Mouse Anti-Human ILT7 (CD85g) antibody (Cat. No. 562355; Right Panel). The two-color flow cytometric dot plots show ILT7 (or Ig isotype control staining) expression patterns of Lin-1-negative, CD123-positive cells with the forward and side-light scattering characteristics of viable PBMC. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Product Details
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BD Pharmingen™
LILRA4; CD85g; ILT7; ILT-7; Immunoglobulin-like transcript 7
Human (QC Testing)
Mouse IgG1, κ
ILT7–FcεRIγ-transfected 2B4 cells
Flow cytometry (Routinely Tested)
5 µl
23547
AB_11154600
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Recommended Assay Procedures

Note: For best staining results, cell samples should be stained and maintained at 4-8°C before flow cytometric analysis.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
562355 Rev. 1
Antibody Details
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17G10.2

The 17G10.2 monoclonal antibody specifically binds to human Immunoglobulin Like Transcript 7 (ILT7), also known as CD85g. The ILT7 protein is encoded by the LILRA4 (Leukocyte Immunoglobulin Like Receptor subfamily A member 4) gene. ILT7 is a member of immunoglobulin-like transcripts (ILT) or leukocyte immunoglobulin-like receptor (LIR) gene family. ILT7 is selectively expressed on plasmacytoid dendrtic cells (pDC) but not on myeloid dendritic cells or other peripheral blood leukocytes. In response to IL-3, IL-3 receptor complex signaling downregulates ILT7 expression by pDC. ILT7 associates with the FcεRIγ signal adapter protein to form a receptor complex that can signal through activation of an immunoreceptor-based tyrosine activation motif (ITAM)-mediated signaling pathway. Signaling through the ILT7-FcεRIγ receptor complex negatively regulates the innate immune functions of pDC. ILT7 crosslinking inhibits TLR responses by pDC such as the stimulated production of type I interferon and other cytokines. Bone marrow stromal cell antigen 2 (BST2) has been identified as a biological ligand for ILT7.

562355 Rev. 1
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
562355 Rev.1
Citations & References
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Development References (3)

  1. Cao W, Bover L, Cho M, et al. Regulation of TLR7/9 responses in plasmacytoid dendritic cells by BST2 and ILT7 receptor interaction.. J Exp Med. 2009; 206(7):1603-14. (Biology). View Reference
  2. Cao W, Bover L. Signaling and ligand interaction of ILT7: receptor-mediated regulatory mechanisms for plasmacytoid dendritic cells. Immunol Rev. 2010; 234(1):163-176. (Biology). View Reference
  3. Cao W, Rosen DB, Ito T, et al. Plasmacytoid dendritic cell-specific receptor ILT7-Fc epsilonRI gamma inhibits Toll-like receptor-induced interferon production.. J Exp Med. 2006; 203(6):1399-405. (Immunogen: Bioassay, Flow cytometry, Inhibition). View Reference
562355 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.