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      Alexa Fluor™ 647 Mouse Anti-Human Chromogranin A
      Alexa Fluor™ 647 Mouse Anti-Human Chromogranin A
      Flow cytometric analysis of Chromogranin A expression in Human neuroblastoma cells.  Cells from the Human SH-SY5Y (Neuroblastoma, ATCC® CRL-2266™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). The cells were then washed, permeabilized with and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either Alexa Fluor™ 647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; dashed line histogram) or Alexa Fluor™ 647 Mouse Anti-Human Chromogranin A antibody (Cat. No. 570072/570153; solid line histogram). The fluorescence histogram showing Chromogranin A expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact SH-SY5Y cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
      Alexa Fluor™ 647 Mouse Anti-Human Chromogranin A
      Flow cytometric analysis of Chromogranin A expression in Human neuroblastoma cells.  Cells from the Human SH-SY5Y (Neuroblastoma, ATCC® CRL-2266™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). The cells were then washed, permeabilized with and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either Alexa Fluor™ 647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; dashed line histogram) or Alexa Fluor™ 647 Mouse Anti-Human Chromogranin A antibody (Cat. No. 570072/570153; solid line histogram). The fluorescence histogram showing Chromogranin A expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact SH-SY5Y cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
      Product Details
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      BD Pharmingen™
      CGA; CgA; CHGA; Chromogranin-A; CMGA; SP-I
      Human (QC Testing)
      Mouse IgG1, κ
      Human Chromogranin A Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      5 µl/test
      1113
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      3. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      9. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
      10. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      11. For U.S. patents that may apply, see bd.com/patents.
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      570153 Rev. 1
      Antibody Details
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      S21-537

      Chromogranin A (CGA) is a member of the granin family of regulated secretory proteins that are found in secretory granules in endocrine and neuroendocrine cells and released in response to extracellular stimulation. Intracellularly, granins are important for targeting peptide hormones and neurotransmitters by their ability to aggregate in the low pH, high calcium environment of the trans-Golgi network. Extracellularly, peptides formed from proteolytic processing of granins regulate hormone secretion. CGA is a prohormone that can be cleaved into several biologically active peptides, such as pancreastatin, β-granin, vasostatin, catestatin, and parastatin. β-granin is an N-terminal fragment of CGA, while pancreastatin and catestatin are processed from the central region of CGA. Cells of the adrenal medulla, anterior pituitary, cerebral cortex as well as beta cells of the pancreas and a variety of tumor cell lines express CGA. The expression of CGA can be used to monitor the pancreatic differentiation of pluripotent stem cells.

      570153 Rev. 1
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      570153 Rev.1
      Citations & References
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      View product citations for antibody "570153" on CiteAb

      Development References (8)

      1. D'Amour KA, Bang AG, Eliazer S, et al . Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol. 2006; 24(12):1481-1483. (Biology). View Reference
      2. Hendy GN, Bevan S, Mattei MG, Mouland AJ. Chromogranin A. Clin Invest Med. 1995; 18(1):47-65. (Biology: Intracellular Staining/Flow Cytometry). View Reference
      3. Kroon E, Martinson LA, Kadoya K, Bang AG, et al. Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo. Nat Biotechnol. 2008; 26(4):443-452. (Biology). View Reference
      4. Loh YP, Cheng Y, Mahata SK, Corti A, Tota B. Chromogranin A and derived peptides in health and disease. J Mol Neurosci. 2012; 48(2):347-356. (Biology). View Reference
      5. Mendieta I, Nuñez-Anita RE, Pérez-Sánchez G, et al. Effect of A549 neuroendocrine differentiation on cytotoxic immune response.. Endocr Connect. 2018; 7(5):791-802. (Clone-specific: Flow cytometry). View Reference
      6. Mouland AJ, Bevan S, White JH, Hendy GN. Human chromogranin A gene. Molecular cloning, structural analysis, and neuroendocrine cell-specific expression. J Biol Chem. 1994; 269(9):6918-6926. (Biology). View Reference
      7. Schulz TC, Young HY, Agulnick AD et al. A scalable system for production of functional pancreatic progenitors from human embryonic stem cells. PLoS ONE. 7(5)(Biology). View Reference
      8. Taylor CV, Taupenot L, Mahata SK. Formation of the catecholamine release-inhibitory peptide catestatin from chromogranin A. Determination of proteolytic cleavage sites in hormone storage granules. Clin Invest Med. 2000; 275(30):22905-22915. (Biology). View Reference
      View All (8) View Less
      570153 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.